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, Volume 38, Supplement 1, pp 60–65 | Cite as

Dihydrolipoic acid protects pancreatic islet cells from inflammatory attack

  • V. Burkart
  • T. Koike
  • H. -H. Brenner
  • Y. Imai
  • H. Kolb
Inflammation

Abstract

In vitro models of pancreatic islet cell inflammation are the lysis of isolated islet cells by activated macrophages or by oxygen radicals released by the endothelial enzyme xanthine oxidase. Dihydrolipoic acid protected islet cells in both systems by different modes of action. Macrophage cytotoxicity towards islet cells, which is nitric-oxide-mediated, was suppressed by 2 h of preincubation of macrophages with lipoic acid. Similarly, 2 h of preincubation sufficed to protect islet cells against enzymatically produced oxygen radicals. Dihydrolipoic acid was found by chemiluminescence assay to scavenge directly such radicals. In macrophages dihydrolipoic acid suppressed the production of nitrite as a measure of nitric oxide release. These results suggest that dihydrolipoic acid is an anti-inflammatory agent which at the same time interferes with nitric oxide release from inflammatory macrophages and protects target cells from oxygen radical attack.

Keywords

Nitric Oxide Nitrite Xanthine Islet Cell Oxygen Radical 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Birkhäuser Verlag 1993

Authors and Affiliations

  • V. Burkart
    • 1
  • T. Koike
    • 1
  • H. -H. Brenner
    • 1
  • Y. Imai
    • 1
  • H. Kolb
    • 1
  1. 1.Diabetes Research Institute at the University of DüsseldorfDüsseldorfGermany

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