Modulation of adherence of coagulase-negative staphylococci to teflon catheters in vitro
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The mechanism of adherence ofStaphylococcus epidermidisto commercially available catheters was studied in vitro in a quantitative assay employing3H-labelled bacteria. It was found that adherence to Teflon catheters was significantly related to the degree of hydrophobicity of the strains. When hydrophobic groups were removed fromStaphylococcus epidermidisby pepsin treatment, adhesion was almost completely abolished. Preincubation of catheters in human serum also caused a 80–90 % reduction of adherence. Preincubation ofStaphylococcus epidermidisin serum similarly decreased adhesion. This effect of serum was mainly due to albumin, while IgG and fibronectin were less effective. Culture ofStaphylococcus epidermidisin subinhibitory concentrations (0.5 MIC) of cephalothin, clindamycin and vancomycin resulted in a 30–80 % reduction in adhesion.
KeywordsCatheter Internal Medicine Albumin Vancomycin Human Serum
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