European Journal of Clinical Microbiology

, Volume 4, Issue 2, pp 170–174 | Cite as

Trafficking ofPseudomonas exotoxin a in mammalian cells

  • C. B. Saelinger
  • R. E. Morris
  • G. Foertsch
Current Topic: Pseudomonas Aeruginosa Infections

Abstract

Experiments designed to elucidate cellular internalization ofPseudomonas aemginosa exotoxin A are described. Inhibition of protein synthesis was used as an index of the biological activity of exotoxin A, and a biotinyl-toxin: avidin-gold system to follow its movement on the ultrastructural level. Addition of amantadine, methylamine and dansylcadaverine to cells enhanced the toxicity of exotoxin A at lower concentrations, while protecting cells at higher concentrations. In general, both sensitive and resistant cell lines responded similarly. Exposure of LM or Vero cells to an acidic extracellular pH did not overcome the protection afforded by ammonium chloride against exotoxin A cytotoxicity. This and other data suggest that sensitive and resistant cells may internalize exotoxin A in a similar manner, the toxin entering the cytosol from a prelysosomal acidic vacuole.

Keywords

Chloride Ammonium Internal Medicine Biological Activity Protein Synthesis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Vieweg Publishing 1985

Authors and Affiliations

  • C. B. Saelinger
    • 1
  • R. E. Morris
    • 2
  • G. Foertsch
    • 1
  1. 1.Department of Microbiology and Molecular GeneticsUniversity of Cincinnati College of MedicineCincinnatiUSA
  2. 2.Department of Anatomy and Cell BiologyUniversity of Cincinnati College of MedicineCincinnatiUSA

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