Calcified Tissue Research

, Volume 23, Issue 1, pp 103–112 | Cite as

Movement of125I albumin and125I polyvinylpyrrolidone through bone tissue fluid

  • Maureen Owen
  • C. R. Howlett
  • J. T. Triffitt
Original Papers
Received:
Accepted:

Summary

The passage of tissue fluid through cortical bone has been investigated using radioactively labelled macromolecules as markers. The results suggest that in the cortex of young rabbit femur the movement of tissue fluid is in the same net direction as blood, mainly from the endosteal to the periosteal surface.

Some albumin is incorporated from extravascular tissue fluid into calcified matrix at sites of bone formation. Polyvinylpyrrolidone, average molecular weight 35,000 is able to pass through extravascular tissue fluid in bone but isnot incorporated into calcified matrix. In rabbits made vitamin D deficient, much less albumin is retained in regions of bone formation than is the case with controls. Albumin adsorbs to the surface of calcium phosphate precipitates and it is suggested that this mechanism may be mainly responsible for its incorporation into bone.

Key-words

Albumin Polyvinylpyrrolidone Tissue fluid Bone 
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References

  1. Anderson, D.W.: Studies of the lymphatic pathways of bone and bone marrow. J. Bone Jt Surg.42A, 716–717 abstr. (1960)Google Scholar
  2. Anker, H.S.: The Biosynthesis of Plasma Proteins. In: The Plasma Proteins, Vol. II (Putman, F.W., ed.) p. 267, New York and London: Academic Press 1960Google Scholar
  3. Brookes, M.: The Blood Supply of Bone. London: Butterworth (1971).Google Scholar
  4. Cooper, R.R., Milgram, J.W., Robinson, R.A.: Morphology of the osteon. J. Bone Jt Surg.48, 1239–1271 (1966)Google Scholar
  5. Courtice, F.C.: Lymph and Plasma Proteins: Barriers to their Movement throughout the Extracellular Fluid. Lymphology4, 1–17 (1971)Google Scholar
  6. Doty, S.B., Schofield, B.H.: In: Calcium, Parathyroid Hormone and the Calcitonins (R.V. Talmage and P.L. Munson, eds.), pp. 351–364, International Congress Series No. 243, Amsterdam: Excerpta Medica 1972Google Scholar
  7. Luk, S.C., Nopajaroonsri, C., Simon, G.T.: The Ultrastructure of Endosteum. J. Ultrastructure Research46, 165–183 (1974)CrossRefGoogle Scholar
  8. Mancini, G., Carbonara, A.O., Heremans, J.F.: Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry2, 235–254 (1965)CrossRefPubMedGoogle Scholar
  9. Michelsen, K.: Pressure relationships in the bone marrrow vascular bed. Acta physiol. scand.71, 12–29 (1967)Google Scholar
  10. Michelsen, K.: Determination of Inulin, Albumin and Erythrocyte Spaces in the bone marrow of rabbits.77, 28–35 (1969)Google Scholar
  11. Nelson, G.E., Kelly, P.J., Peterson, F., Janes, J.: Blood supply of the human tibia. J. Bone Jt Surg.42A, 625–636 (1960)Google Scholar
  12. Neuman, W.F., Ramp, W.K.: The concept of a bone membrane. In: Cellular mechanisms for calcium transfer and homeostasis (G. Nichols and R.H. Wasserman, eds.), pp. 197–209. New York: Academic Press 1971Google Scholar
  13. Owen, M.: Cell population kinetics of an osteogenic tissue. J. Cell Biol.19, 19–32 (1963)CrossRefPubMedGoogle Scholar
  14. Owen, M., Triffitt, J.T.: Extravascular albumin in bone tissue. J. Physiol.257, 293–307 (1976)PubMedGoogle Scholar
  15. Owen, M., Triffitt, J.T., Melick, R.A.: Albumin in bone. In: Hard Tissue Growth, Repair and Remineralisation (K. Elliott and D.W. Fitzsimmons, eds) Ciba Foundation Symposium 11, pp. 263–293, Amsterdam: Elsevier, Excerpta Medica, North-Holland 1973Google Scholar
  16. Perkin-Elmer: In: Analytical Methods for Atomic Absorption Spectrophotometry, Norwalk, Connecticut, U.S.A. (1968)Google Scholar
  17. Pritchard, J.J.: A cytological and histochemical study of bone and cartilage formation in the rat. J. Anat.86, 259–269 (1952)PubMedGoogle Scholar
  18. Rhinelander, F.W.: Circulation in Bone. In: The Biochemistry and Physiology of Bone (G.H. Bourne, ed.), pp. 1–77, New York: Academic Press 1972Google Scholar
  19. Seliger, W.G.: Tissue, fluid movement in compact bone. Anat. Rec.166, 247–255 (1970)CrossRefPubMedGoogle Scholar
  20. Shim, S-S., Hawk, H.E., Yu, W.Y.: The relationship between blood flow and marrow cavity pressure of bone. Surgery135, 353–360 (1972)Google Scholar
  21. Szabo, G., Magyar, Z., Molnar, G.: Lymphatic and venous transport of colloids from the tissues. Lymphology6, No. 2, 69–78 (1973)PubMedGoogle Scholar
  22. Triffitt, J.T., Owen, M.: Studies on bone matrix glycoproteins. biochem. J.136, 125–134 (1973)PubMedGoogle Scholar
  23. Yoffey, J.M.: Structural peculiarities of the blood vessels of the bone marrow. Bibl. Anat. (Basel),7, 298–303 (1965)Google Scholar
  24. Yoffey, J.M., Courtice, F.C.: Lymphatics, Lymph and Lymphoid Tissue, p. 12, London: Edward Arnold 1956Google Scholar
  25. Zamboni, L., Pease, D.C.: The vascular bed of red bone marrow. J. Ultrastruct. Res.5, 65–85 (1961)CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • Maureen Owen
    • 1
  • C. R. Howlett
    • 1
  • J. T. Triffitt
    • 1
  1. 1.Bone Research Laboratory, Nuffield Department of Orthopaedic SurgeryUniversity of Oxford, Nuffield Orthopaedic CentreOxfordUK

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