Electron microscope study of the interaction of epibiontic bacteria withChromatium minus in natural habitats
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Epibiontic cells on the surface of the photosynthetic purple sulfur bacteriumChromatium minus, collected several times during the year from 3 different Spanish lakes, were examined using scanning and transmission electron microscopy. The cells attached to theC. minus cell wall by an electron-dense pad, but did not enter the cell. They were ovoidal (about 0.6Μm wide) when free, and slightly curved rods (0.3×0.6Μm) when undergoing division. Division only occurred when cells remained attached toChromatium. A septum was formed, resulting in 2 or 3 curved rods surrounded by a common capsule. Detached daughter cells became ovoidal.
The host ultrastructure changed as a result of epibiontic attachment, showing symptoms of cellular degradation. Simultaneously, plaques could be detected on cell lawns formed spontaneously upon cell sedimentation from field samples.
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- 2.Anderson TF (1951) Techniques for the preservation of three-dimensional structure in preparing specimens for the electron microscope. Trans NY Acad Sci 13:130–135Google Scholar
- 4.Coder DM, Starr MP (1978) Antagonistic association of the chlorellavorus bacterium (“Bdellovibrio” chlorellavorus) withChlorella vulgaris. Current Microbiology 1:59–64Google Scholar
- 6.Gromov BV, Mamkayeva KA (1972) Electron microscopic examination ofBdellovibrio chlorellavorus parasitism on cells of the green algaChlorella vulgaris. Tsitologiya 14:256–260 (In Russian)Google Scholar
- 7.Gromov BV, Mamkayeva KA (1980) Proposal of a new genusVampirovibrio for chlorellavorus bacteria previously assigned toBdellovibrio. Mikrobiologya 49:165–167 (In Russian)Google Scholar
- 8.Guerrero R, Montesinos E, Esteve I, Abellà C (1980) Physiological adaptation and growth of purple and green sulfur bacteria in a meromictic lake (Vilar) as compared to a holomictic lake (Sisó). In: Dokulil M, Metz H, Jewson D (eds) Shallow lakes. Developments in hydrobiology, Vol 3. W Junk Publishers, The Hague, pp 161–171Google Scholar
- 9.Huang JC, Starr MP (1973) Possible enzymatic bases of bacteriolysis by bdellovibrios. Arch Microbiol 89:147–167Google Scholar
- 11.Larkin JM (1980) Isolation ofThiothrix in pure culture and observation of a filamentous epiphyte onThiothrix. Current Microbiology 4:155–158Google Scholar
- 12.Paerl HW (1976) Specific associations of the bluegreen algaeAnabaena andAphanizomenon with bacteria in freshwater blooms. J Phycol 12:431–435Google Scholar
- 17.Stolp H, Petzold H (1962) Untersuchungen über einen obligat parasitischen Microorganismus mit lytischer AktivitÄt fürPseudomonas-Bakterien. Phytopathol Zeitschrift 45:364–390Google Scholar
- 18.Stolp H (1973) The bdellovibrios: bacterial parasites of bacteria. Annu Rev Phytopathol 11:53–76Google Scholar
- 21.Van Gemerden H (1980) Survival ofChromatium vinosum at low light intensities. Arch Microbiol 125:115–121Google Scholar