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Microbial Ecology

, Volume 5, Issue 2, pp 139–146 | Cite as

Inhibitory effects of copper on bacteria related to the free ion concentration

  • L. P. T. M. Zevenhuizen
  • J. Dolfing
  • E. J. Eshuis
  • Ineke J. Scholten-Koerselman
Article

Abstract

Cu2+ ion determinations were carried out in complex and in inorganic salts-glycerol media, to which increasing amounts of Cu(II) had been added, with the ion-specific Cu(II)-Selectrode. Likewise, complexing capacity of bacterial suspensions was estimated by titration with CuSO4.

Copper-sensitive bacteria, e.g.,Klebsiella aerogenes, were inhibited in their growth and survival in the range of 10−8–10−6 M Cu2+ ion concentrations. In copper-buffered complex media, high copper loads could be tolerated, as growth proceeded with most of the copper bound to medium components. In low-complexing mineral salts media, in which high Cu2+ ion concentrations exist at low copper loads, there was competition of Cu2+ for binding sites of the cells. Total allowed copper was then determined by the ratio of copper to biomass.

Copper-resistant bacteria could be isolated from a stock solution of CuSO4, containing 100 ppm Cu(II). They were of thePseudomonas type and showed a much higher tolerance towards Cu2+, up to 10−3 M.

Keywords

Biomass CuSO4 Klebsiella Mineral Salt Salt Medium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 1979

Authors and Affiliations

  • L. P. T. M. Zevenhuizen
    • 1
  • J. Dolfing
    • 1
  • E. J. Eshuis
    • 1
  • Ineke J. Scholten-Koerselman
    • 1
  1. 1.Laboratory of MicrobiologyAgricultural UniversityWageningenThe Netherlands

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