Microbial Ecology

, Volume 6, Issue 3, pp 277–280

Aerotaxis and chemotaxis ofAzospirillum brasilense: A note

  • Yaacov Okon
  • Lutfu Cakmakci
  • Israel Nur
  • Ilan Chet


Azospirillum brasilense was attracted to capillaries containing either phosphate buffer, distilled water, or saline. The number of bacteria in these capillaries was 3−4×104, after 1 h of incubation. In the presence of phosphate buffer + attractants, the number of cells accumulated in the capillary increased only to 5×104–1.1×105 cells. It was not possible, therefore, to measure chemotaxis inA. brasilense as distinct from aerotaxis by the capillary method. Chemotaxis was observed in semi-solid agar plates and was determined by a growth band oriented towards the attractant. Positive chemotactic response was obtained with peptone, tryptone, yeast extract, amino acids, organic acids, arabinose and galactose.


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  1. 1.
    Adler, J.: Chemotaxis in bacteria. Science153, 708–716 (1966)PubMedGoogle Scholar
  2. 2.
    Adler, J.: A method for measuring chemotaxis and use of the method to determine optimum conditions for chemotaxis byEscherichia coli. J. Gen. Microbiol.74, 77–91 (1973)PubMedGoogle Scholar
  3. 3.
    Caraway, B. H., and N. R. Krieg: Aerotaxis ofSpirillum volutans. Can. J. Microbiol.20, 1367–1377 (1974)Google Scholar
  4. 4.
    Chet, I., Y. Zilberstein, and Y. Henis: Chemotaxis ofPseudomonas lachrymans to plant extracts and to water droplets collected from the leaf surfaces of resistant and susceptible plants. Physiol. Plant Pathol.3, 473–479 (1973)Google Scholar
  5. 5.
    Currier, W. W., and G. A. Strobel: Chemotaxis ofRhizobium spp. to plant root exudates. Plant Physiol.57, 820–823 (1976)Google Scholar
  6. 6.
    Neyra, C. A., and J. Dobereiner: Nitrogen fixation in grasses. Adv. Agron.29, 1–38 (1977)Google Scholar
  7. 7.
    Okon, Y., S. L. Albrecht, and R. H. Burris: Factors affecting growth and nitrogen fixation ofSpirillum lipoferum. J. Bacteriol.127, 1248–1254 (1976)PubMedGoogle Scholar
  8. 8.
    Okon, Y., S. L. Albrecht, and R. H. Burris: Methods for growingSpirillum lipoferum and for counting it in pure culture and in association with plants. Appl. Environ. Microbiol.33, 85–88 (1976)Google Scholar
  9. 9.
    Okon, Y., J. P. Houchins, S. L. Albrecht, and R. H. Burris: The growth ofSpirillum lipoferum at constant partial pressures of oxygen and the properties of its nitrogenase in cell free extracts. J. Gen. Microbiol.98, 87–93 (1977)PubMedGoogle Scholar
  10. 10.
    Rovira, A. D.: Plant root exudates. Bot. Rev.35, 35–57 (1969)Google Scholar
  11. 11.
    Tarrand, J. J., N. R. Krieg, and J. Dobereiner: A taxonomic study of theSpirillum lipoferum group, with descriptions of a new genus,Azospirillum lipoferum (Bijerinck) comb. nov. andAzospirillum brasilense sp. nov. Can. J. Microbiol.24, 967–980 (1978)PubMedGoogle Scholar

Copyright information

© Springer-Verlag New York Inc 1980

Authors and Affiliations

  • Yaacov Okon
    • 1
  • Lutfu Cakmakci
    • 1
  • Israel Nur
    • 1
  • Ilan Chet
    • 1
  1. 1.Department of Plant Pathology and Microbiology, Faculty of AgricultureHebrew University of JerusalemRehovotIsrael
  2. 2.Dept. of Agricultural Microbiology, Faculty of AgricultureUniversity of AnkaraAnkaraTurkey

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