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Calcified Tissue Research

, Volume 8, Issue 1, pp 87–95 | Cite as

The effect of pyrophosphate infusion on the response of the thyroparathyroidectomized rat to parathyroid hormone and adenosine-3′,5′-cyclic monophosphate

  • Allyn Delong
  • Joel Feinblatt
  • Howard Rasmussen
Original Papers

Abstract

The infusion of inorganic pyrophosphate at a rate of 15 μmoles/h into thyroparathyroidectomized rats blocked the effects of parathyroid hormone infusion upon plasma calcium and phosphate, and upon the renal excretion of calcium, phosphate, hydroxyproline-containing peptides, and cyclic 3′5′ AMP. In contrast, pyrophosphate infusion caused no significant change in the effects of cyclic 3′5′ AMP infusion upon plasma calcium and phosphate, or upon the urinary excretion of calcium, phosphate, and hydroxyproline-containing peptides.

Key words

Pyrophosphate Cyclic 3′5′-AMP Parathyroid Adenyl cyclase Bone Resorption 

Résumé

L'injection de pyrophosphate inorganique, à la concentration de 15 μmoles/h, au niveau de rats thyro-parathyréodectomisés, inhibe les effects de l'hormone parathyroidienne injectée, sur le calcium et le phosphate plasmatique et sur l'excrétion rénale du calcium, du phosphate, des peptides contenant l'hydroxyproline et de l'AMP 3′5′ cyclique. Inversement, l'injection de pyrophosphate ne provoque pas de changements significatifs sur les effects d'injection d'AMP 3′5′ cyclique sur le calcium et le phosphate du plasma, ou sur l'excrétion urinaire de calcium, phosphate et peptides contenant de l'hydroxyproline.

Zusammenfassung

Wurde thyroparathyreoidektomierten Ratten anorganisches Pyrophosphat mit einer Geschwindigkeit von 15 μMol/Std infundiert, so blockierte dies die Wirkung der gleichzeitig laufenden Parathormoninfusion auf Plasmacalcium und-phosphat, sowie auf die renale Ausscheidung von Calcium, Phosphat, Hydroxyprolin-haltigen Peptiden und cyclischem 3′,5′-AMP. Hingegen bewirkte die Pyrophosphatinfusion keine signifikante Veränderung der Wirkung einer Infusion von zyklischem 3′,5′-AMP auf Plasmacalcium und-phosphat oder auf die Ausscheidung im Urin von Calcium, Phosphat und Hydroxyprolin-haltigen Peptiden.

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Copyright information

© Springer-Verlag 1971

Authors and Affiliations

  • Allyn Delong
  • Joel Feinblatt
  • Howard Rasmussen
    • 1
  1. 1.Department of BiochemistryUniversity of PennsylvaniaPhiladelphiaUSA

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