Advertisement

Experientia

, Volume 36, Issue 1, pp 37–38 | Cite as

A logical approach to the isolation of lactate dehydrogenase isozyme X from human tests: A general rationale for the isolation of homotetrameric LDH isozymes

  • P. Toowicharanont
  • J. Svasti
Specialia
  • 17 Downloads

Summary

Immunoadsorbent and oxamate-Sepharose chromatography were used to isolate electrophoretically homogeneous LDH-X from human testes with a final specific activity of 125 IU/mg and good yields: other applications of this approach are discussed.

Keywords

Chromatography Lactate Lactate Dehydrogenase General Rationale Good Yield 

References

  1. 2.
    A. Blanco and W. H. Zinkham, Science139, 601 (1963).CrossRefGoogle Scholar
  2. 3.
    E. Goldberg, Science139, 602 (1963).CrossRefGoogle Scholar
  3. 4.
    W. H. Zinkham, A. Blanco and L. J. Clowry, Ann. N.Y. Acad. Sci.121, 571 (1964).CrossRefGoogle Scholar
  4. 5.
    E. Goldberg, J. biol. Chem.247, 2044 (1972).PubMedGoogle Scholar
  5. 6.
    E. Goldberg, Proc. nat. Acad. Sci., USA68, 349 (1971).CrossRefGoogle Scholar
  6. 7.
    E. Goldberg and J. Lerum, Science176, 686 (1972).CrossRefGoogle Scholar
  7. 8.
    J. Lerum and E. Goldberg, Biol. Reprod.11, 108 (1974).CrossRefGoogle Scholar
  8. 9.
    J. Svasti and S. Viriyachai, in: Isozymes, vol. 2, p. 113. Ed. C. L. Markert, Academic Press, New York 1975.CrossRefGoogle Scholar
  9. 10.
    A. H. J. Kolk, L. Van Kuyk and B. Boettcher, Biochem J.173, 767 (1978).CrossRefGoogle Scholar
  10. 11.
    J. Svasti and S. Viriyachai, J. Sci. Soc. Thailand1, 57 (1975).CrossRefGoogle Scholar
  11. 12.
    K. Rajewsky, Biochim. biophys. Acta121, 51 (1966).CrossRefGoogle Scholar
  12. 13.
    J. B. Fleischman, R. H. Pain and R. R. Porter, Archs Biochem. Biophys., suppl.1, 174 (1972).Google Scholar
  13. 14.
    M. Wilchek, V. Bocchini, M. Becker and D. Givol, Biochemistry10, 2828 (1971).CrossRefGoogle Scholar
  14. 15.
    P. O'Carra and S. Barry, FEBS Lett.21, 281 (1972).CrossRefGoogle Scholar
  15. 16.
    J. F. Burd, M. Usategui-Gomez, A. Fernandez de Castro, N. S. Mhatre and F. M. Yeager, Clinica chim. Acta46, 205 (1973).CrossRefGoogle Scholar
  16. 17.
    E. Goldberg, J. exp. Zool.186, 274 (1973).CrossRefGoogle Scholar

Copyright information

© Birkhäuser Verlag 1980

Authors and Affiliations

  • P. Toowicharanont
    • 1
  • J. Svasti
    • 1
  1. 1.Department of Biochemistry, Faculty of ScienceMahidol UniversityBangkok 4Thailand

Personalised recommendations