Interleukin-4 in mononuclear cells and histamine release in peripheral blood from an atopic patientin vitro
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Abstract
In this study, we investigated whether stimulated and unstimulated peripheral blood mononuclear cells (PBMNCs) from atopic and healthy donors produce interleukin-4 (IL-4)in vitro in response to various stimulants. Furthermore we examined if histamine release in leucocyte incubates from the same donors corresponded to the induced IL-4 production. We found a significantly increased IL-4 production in both stimulated and unstimulated PBMNCs during the first 48 h in culture (p<0.01,n=18 cultures). Comparing atopic to healthy PBMNCs no significant differences in the IL-4 production could be detected (p>0.1). The kinetics of the induced IL-4 production showed a maximum after 24 h in culture in the stimulated atopic and healthy samples. Both mitogens PHA and PWM induced a similar pattern of IL-4 production. As for histamine liberation, it was significantly increased in the atopic incubates compared with the healthy samples. From these data it can be suggested that PBMNCs from atopic and healthy donors are able to produce small amounts (3–8 pg/ml) of IL-4in vitro, but this IL-4 production does not correspond to elevated histamine release by the atopic patient's leucocytesin vitro.
Keywords
Histamine Similar Pattern Mononuclear Cell Peripheral Blood Mononuclear Cell Healthy DonorReferences
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