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Genotypic homogeneity of nosocomialPseudomonas aeruginosa O12 strains demonstrated by analysis of protein profiles, DNA fingerprints and rRNA gene restriction patterns

  • F. Grattard
  • O. G. Gaudin
  • B. Pozzetto
  • A. Ros
  • A. D. Mbida
Notes

Abstract

The spread in Europe of a single multiresistant strain ofPseudomonas aeruginosa serotype O12 has been suggested. This bacterium was responsible for a nosocomial outbreak in our hospital in 1988–1989. Three different epidemiological methods were used to analyze 30 strains isolated during five consecutive years. Protein profile analysis and chromosomal DNA fingerprinting with four different enzymes revealed closely related patterns. rRNA gene restriction fragment length analysis performed with a digoxigenin-labelled probe showed identical hybridization patterns with four to six bands according to the endonuclease used. Combination of the three typing methods showed genotypic homogeneity of thesePseudomonas aeruginosa O12 strains, despite a relative increase in their antibiotic resistance.

Keywords

Protein Profile Restriction Pattern Hybridization Pattern Related Pattern Length Analysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Pitt TL Epidemiological typing ofPseudomonas aeruginosa. European Journal of Clinical Microbiology & Infectious Diseases 1988, 7: 238–247.Google Scholar
  2. 2.
    Veron M, Descamps P, Daoulas F, Loulergue J, Weber M, Mallet MN, Lagrange P, Dabernat H, Lemeland JF, Morel CI, Philippon A, Didion J, Vincent P Etude multicentrique de la sensibilité dePseudomonas aeruginosa à six beta-lactamines. Médecine et Maladies Infectieuses 1987, 17: 401–411.Google Scholar
  3. 3.
    Pitt TL, Livermore DM, Pitcher D, Vatopoulos AC, Legakis NJ Multiresistant serotype O12Pseudomonas aeruginosa: evidence for a common strain in Europe. Epidemiology and Infection 1989, 103: 565–576.PubMedGoogle Scholar
  4. 4.
    Walia S, Madhavan T, Williamson T, Kaiser A, Tewari R Protein patterns, serotyping and plasmid DNA profiles in the epidemiological fingerprinting ofPseudomonas aeruginosa. European Journal of Clinical Microbiology & Infectious Diseases 1988, 7: 248–255.Google Scholar
  5. 5.
    Sambrook J, Fritsch EF, Maniatis R Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 1989.Google Scholar
  6. 6.
    Brosius J, Ullrich A, Raker MA, Gray A, Dull TJ, Gutell RR, Noller HF Construction and fine mapping of recombinant plasmids containing therrnB ribosomal RNA operon ofE. coli. Plasmid 1981, 6: 112–118.PubMedGoogle Scholar
  7. 7.
    Pitt TL, Livermore DM, Miller G, Vatopoulos A, Legakis NJ Resistance mechanisms of multiresistant O12Pseudomonas aeruginosa isolated in Europe. Journal of Antimicrobial Chemotherapy 1990, 26: 319–328.PubMedGoogle Scholar
  8. 8.
    McGeer A, Low DE, Penner J, NG J, Golman G, Simor AE Use of molecular typing to study the epidemiology ofSerratia marcescens. Journal of Clinical Microbiology 1990, 28: 55–58.PubMedGoogle Scholar
  9. 9.
    Bruce KD, Jordens JZ Characterization of noncapsulateHaemophilus influenzae by whole-cell polypeptide profiles, restriction endonuclease analysis, and rRNA gene restriction patterns. Journal of Clinical Microbiology 1991, 29: 291–296.PubMedGoogle Scholar
  10. 10.
    Stull TL, LiPuma JJ, Edlind TD A broad-spectrum probe for molecular epidemiology of bacteria: ribosomal RNA. Journal of Infectious Diseases 1988, 157: 280–286.PubMedGoogle Scholar
  11. 11.
    Loutit JS, Tompkins LS Restriction enzyme and Southern hybridization analyses ofPseudomonas aeruginosa strains from patients with cystic fibrosis. Journal of Clinical Microbiology 1991, 29: 2897–2900.PubMedGoogle Scholar
  12. 12.
    Grimont F, Grimont PAD Ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools. Annales de l'Institut Pasteur 1986, 137B: 165–175.PubMedGoogle Scholar
  13. 13.
    Bingen EH, Denamur E, Lambert-Zechovsky NY, Bourdois A, Mariani-Kurkdjian P, Cezard JP, Navarro J, Elion J DNA restriction fragment length polymorphism differentiates crossed from independent infections in nosocomialXanthomonas maltophilia bacteremia. Journal of Clinical Microbiology 1991, 29: 1348–1350.PubMedGoogle Scholar
  14. 14.
    LiPuma JJ, Dasen SE, Nielson DW, Stern RG, Stull TL Person-to-person transmission ofPseudomonas cepacia between patients with cystic fibrosis. Lancet 1990, ii: 1094–1096.Google Scholar
  15. 15.
    Denamur E, Picard B, Goullet PH, Bingen E, Lambert N, Elion J Complexity ofPseudomonas aeruginosa infection in cystic fibrosis: combined results from esterase electrophoresis and rRNA restriction fragment length polymorphism analysis. Epidemiology and Infection 1991, 106: 531–539.PubMedGoogle Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1993

Authors and Affiliations

  • F. Grattard
    • 1
  • O. G. Gaudin
    • 1
  • B. Pozzetto
    • 1
  • A. Ros
    • 1
  • A. D. Mbida
    • 1
  1. 1.Department of Bacteriology-Virology, Faculty of Medicine Jacques LisfrancUniversity of Saint EtienneSaint Etienne Cédex 2France

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