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, Volume 34, Issue 1–2, pp 197–199 | Cite as

Anin vitro model system to evaluate pulmonary macrophage, endothelial cell, and neutrophil interactions

  • S. D. Sharma
  • M. A. Breider
  • T. W. J. Olchowy
Pulmonary Diseases
  • 22 Downloads

Abstract

Pasteurella haemolytica, the cause of fibrinous pleuropneumonia in cattle, produces extensive microvascular endothelial cell (EC) damage. We have developed anin vitro model system to study the inflammatory process of this disease involving the interaction of pulmonary alveolar macrophages (AM), neutrophils (PMN), and EC. Bovine EC are grown to confluency in 24 well tissue culture plates. To mimic the vascular component, 106 PMN are later added to the EC monolayers. Bovine AM are plated onto millicell inserts and placed into the wells containing EC and PMN. The millicell insert serves as a semi-permeable barrier between AM and EC, allowing the exchange of only diffusible material. Preliminary work demonstrates thatP. haemolytica stimulated AM resulted in EC damage presumably due to both soluble lipopolysaccharide and AM secreted products.

Keywords

Endothelial Cell Alveolar Macrophage Tissue Culture Plate Microvascular Endothelial Cell Anin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Birkhäuser Verlag 1991

Authors and Affiliations

  • S. D. Sharma
    • 1
  • M. A. Breider
    • 1
  • T. W. J. Olchowy
    • 2
  1. 1.Dept. of PathobiologyColl. Vet. Med., Univ. TennesseeKnoxville
  2. 2.Dept. of Rural PracticeColl. Vet. Med., Univ. TennesseeKnoxville

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