Experientia

, Volume 50, Supplement 1, pp 75–79 | Cite as

Naja siamensis, a cryptic species of venomous snake revealed by mtDNA sequencing

  • W. Wüster
  • R. S. Thorpe
Research Articles

Abstract

Because of possible variation in venom composition, an understanding of venomous snake systematics is of great importance for the optimization of antivenom treatment of snakebite patients. Intraspecific variation in the morphology of many venomous snakes complicates the definition and indentification of some species when allopatric populations are involved. Selectively neutral or near-neutral mtDNA sequences can reveal evolutionary relationships obscured by ecogenetically-caused morphological variation. We use comparative sequencing of the cytochrome oxidase subunit 1 gene to reveal the existence of a widespread, cryptic species of spiting cobra from southeast Asia. This species,Naja siamensis, is widely sympatric with other Asiatic cobra species. This may be of considerable medical significance, and calls for further research into venom composition in Asiatic cobras.

Key words

MtDNA sequencing molecular systematics Naja Serpentes Elapidae cryptic species snakebite antivenom 

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References

  1. 1.
    Swaroop, S., and Grab, B., Bull. Wld Hlth Org.10 (1954) 35.Google Scholar
  2. 2.
    Minton, S. A., and Minton M. R., Venomous Reptiles Scribners, New York 1980.Google Scholar
  3. 3.
    Warrell, D. A., in: Natural toxins — Animal, plant and microbial, p. 25, Ed. J. B. Harris, Clarendon, Oxford 1986.Google Scholar
  4. 4.
    Looareesuwan, S., Viravan, C., and Warrell, D. A., Trans. R. Soc. trop. Med. Hyg.82 (1988) 930.PubMedGoogle Scholar
  5. 5.
    Viravan, C., Veeravat, U., Warrell, M. J., Theakston, R. D. G., and Warrell, D. A., Am. J. trop. Med. Hyg.35 (1986) 173.PubMedGoogle Scholar
  6. 6.
    Viravan, C., Looareesuwan, S., Kosakarn, W., Wuthiekanun, V., McCarthy, C. J., Stimson, A. F., Bunnag, D., Harinasuta, T., and Warrell, D. A., Trans. R. Soc. trop. Med. Hyg.86 (1992) 100.PubMedGoogle Scholar
  7. 7.
    Sawai, Y., Koba, K., Okonogi, T., Mishima, S., Kawamura, Y., Chinzei, H., Abu-Bakar, I., Devaraj, T., Phong-Aksara, S., Purannanda, C., Salafranca, E. S., Sumpaico, J. S., Tseng, C., Taylor, J. F., Wu, C., and Kuo, T., Jap. J. exp. Med.42 (1972) 283.PubMedGoogle Scholar
  8. 8.
    Watt, G., Padre, L., Tuazon, M. L., and Hayes, C. G., Am. J. trop. Med. Hyg.37 (1987) 636.PubMedGoogle Scholar
  9. 9.
    Watt, G., Padre, L., Tuazon, M. L., Theakston, R. D. G., and Laughlin, L. W., Am. J. trop. Med. Hyg.39 (1988) 306.PubMedGoogle Scholar
  10. 10.
    Wüster, W., and Thorpe, R. S., Biol. J. Linn. Soc.36 (1989) 391.Google Scholar
  11. 11.
    Wüster, W., and Thorpe, R. S., in: Vertebrates in the tropics, p. 333 Eds. G. Peters, and R. Hutterer, Museum A. Koenig, Bonn 1990.Google Scholar
  12. 12.
    Wüster, W., and Thorpe, R. S., Experientia47 (1991) 205.PubMedGoogle Scholar
  13. 13.
    Wüster, W., and Thorpe, R. S., Herpetologica48 (1992) 69.Google Scholar
  14. 14.
    Wüster, W., and Thorpe, R. S., Herpetologica48 (1992) 424.Google Scholar
  15. 15.
    Smith, M. A., Fauna of British India: Reptilia and Amphibia vol. III, Serpentes, Taylor and Francis, London 1943.Google Scholar
  16. 16.
    Taylor, E. H., Kans. Univ Sci. Bull.45 (1965) 609.Google Scholar
  17. 17.
    Lingenhöle, S., and Trutnau, L., Herpetofauna11 (1989) 6.Google Scholar
  18. 18.
    Tumwipat, B., and Nutaphand, W., The treatment of poisonous snakebite victims and the poisonous snakes of Thailand] (in Thai). Peekhanet Print Shop, Bangkok 1982.Google Scholar
  19. 19.
    Cox, M. J., The Snakes of Thailand of Their Husbandry, Krieger, Malabar 1991.Google Scholar
  20. 20.
    Thorpe, R. S., Boll. Zool.54 (1987) 3.Google Scholar
  21. 21.
    Thorpe, R. S., Brown, R. P., Malhotra, A., and Wüster, W., Boll. Zool.58 (1991) 329.Google Scholar
  22. 22.
    Wilson, A. C., Cann, R. L., Carr, S. M., George, M., Gyllensten, U. B., Helm-Bychowski, K. M., Higuchi, R. M., Palumbi, S. R., Prager, E. M., Sage, R. D., and Stoneking, M., Biol. J. Linn. Soc.26 (1985) 375.Google Scholar
  23. 23.
    DeSalle, R., and Templeton, A. R., Evolution42 (1988) 1076.Google Scholar
  24. 24.
    Thorpe, R. S., McGregor, D. P., Cumming, A. M., and Jordan, W. C., Evolution (1993) in press.Google Scholar
  25. 25.
    Saiki, R.K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., and Erlich, H. A., Science239 (1988) 487.PubMedGoogle Scholar
  26. 26.
    Kocher, T. D., Thomas, W. K., Meyer, A., Edwards, S. V., Pääbo, S., Villablanca, F. X., and Wilson, A. C., Proc. natl Acad. Sci. USA86 (1989) 6196.PubMedGoogle Scholar
  27. 27.
    Sanger, F., Nicklen, S., and Coulson, A. R., Proc. natl Acad. Sci. USA74 (1977) 5463.PubMedGoogle Scholar
  28. 28.
    Anderson, S., Bankier, A. T., Barrell, B. G., de Bruijin M. H. L., Coulson, A. R., Drouin, J., Eperon, I. C., Nierlich, D. P., Roe, B. A., Sanger, F., Schrier, P. H., Smith, A. J. H., Staden, R., and Young, I. G., Nature290 (1981) 457.PubMedGoogle Scholar
  29. 29.
    Knight, A., and Mindell, D. P., Syst. Biol.42 (1993) 18.Google Scholar
  30. 30.
    Meier, J., Toxicon24 (1986) 41.PubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag Basel 1994

Authors and Affiliations

  • W. Wüster
    • 1
  • R. S. Thorpe
    • 1
  1. 1.School of Biological SciencesUniversity College of North WalesGwyneddWales, UK

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