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, Volume 18, Issue 1–2, pp 96–99 | Cite as

Forskolin inhibits the release of histamine from human basophils and mast cells

  • G. Marone
  • M. Columbo
  • M. Triggiani
  • S. Vigorita
  • S. Formisano
Histamine Release

Abstract

We found that forskolin (10−7 to 3×10−5M) caused dose-related inhibition of antigen-induced histamine release from human basophil leukocytes. The dose-response inhibition curve was paralleled by a forskolin-induced increase in cyclic AMP (cAMP) levels in human leukocyte preparations. The kinetics of inhibition of histamine release and of the increase in leukocyte cAMP were the same.

In a second series of experiments we evaluated the effect of forskolin on antigen-induced histamine release from chopped human lung passively sensitized with serum from an allergic patient. Forskolin (10−7 to 3×10−5M) dosedependently inhibited the release of histamine from human lung mast cells. Thus forskolin appears to modulate the release of mediators of the immediate hypersensitivity reaction, presumably through activation of adenylate cyclase in human basophils and mast cells.

Keywords

Mast Cell Histamine Human Lung Hypersensitivity Reaction Adenylate Cyclase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. [1]
    M. Ricci andG. Marone,Progress in Clinical Immunology. The Role of Chemical Mediators and Cellular Interactions, Karger, Basel, 1983.Google Scholar
  2. [2]
    T. Ishizaka, D.H. Conrad, E.S. Schulman, A.R. Sterk andK. Ishizaka,Biochemical analysis of initial triggering events of IgE-mediated histamine release from human lung mast cells, J. Immun.130, 2357–2362 (1983).PubMedGoogle Scholar
  3. [3]
    G. Marone,The role of basophils and mast cells in the pathogenesis of pulmonary diseases, Int. Archs. Allergy Appl. Immun.76, suppl. 1, 70–82 (1985).Google Scholar
  4. [4]
    G. Marone, S.R. Findlay andL.M. Lichtenstein,Adenosine receptor on human basophils: modulation of histamine release, J. Immun.123, 1473–1477 (1979).PubMedGoogle Scholar
  5. [5]
    G. Marone, S. Vigorita, C. Antonelli, G. Torella, A. Genovese andM. Condorelli,Evidence for an adenosine A 2/R a receptor on human basophils, Life Sci.36, 339–345 (1985).CrossRefPubMedGoogle Scholar
  6. [6]
    G. Marone, G. Ambrosio, D. Bonaduce, A. Genovese, M. Triggiani andM. Condorelli,Inhibition of IgE-mediated histamine release from human basophils and mast cells by fenoterol, Int. Archs. Allergy Appl. Immun.74, 356–361 (1984).Google Scholar
  7. [7]
    S.V. Bhat, B.S. Bajwa, H. Dornauer andN.J. de Souza,Structures and stereochemistry of new labdane diterpenoids from Coleus forskohliibriq, Tetrahedron Lett.19, 1669–1672 (1977).CrossRefGoogle Scholar
  8. [8]
    K.B. Seamon, W. Padget andJ.W. Daly,Forskolin: unique diterpene activator of adenylate cyclase in membranes and in intact cells, Proc. Natl. Acad. Sci. USA78, 3363–3367 (1981).PubMedGoogle Scholar
  9. [9]
    W. Kreutner, R. Chapman, B.A. Gulbenkian andS. Tozzi,Bronchodilator and anti-allergy activity of forskolin (abstr.), J. Allergy Clin. Immun.73, 130 (1984).Google Scholar
  10. [10]
    B.J. Undem andC.K. Buckner,Forskolin inhibits antigen-induced histamine release from guinea-pig lung (abstr.), Fed. Proc.43, 369 (1984).Google Scholar
  11. [11]
    G. Marone, M. Columbo, L. Soppelsa andM. Condorelli,The mechanism of basophil histamine release induced by pepstatin A, J. Immun.133, 1542–1546 (1984).PubMedGoogle Scholar
  12. [12]
    D.W. MacGlashan Jr. andL.M. Lichtenstein,The purification of human basophils, J. Immun.124, 2519–2521 (1980).PubMedGoogle Scholar
  13. [13]
    M.P. Dubey, R.C. Srimal, S. Nityanand andB.N. Dhawan,Pharmacological studies on coleonol, a hypotensive diterpene from Coleus forskohlii, J. Ethnopharmacol.3, 1–13 (1981).CrossRefPubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag 1986

Authors and Affiliations

  • G. Marone
    • 1
    • 2
  • M. Columbo
    • 1
    • 2
  • M. Triggiani
    • 1
    • 2
  • S. Vigorita
    • 1
    • 2
  • S. Formisano
    • 1
    • 2
  1. 1.Department of MedicineUniversity of Naples II School of MedicineNaplesItaly
  2. 2.Department of Molecular and Cellular Biology and PathologyUniversity of Naples II School of MedicineNaplesItaly

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