Forskolin inhibits the release of histamine from human basophils and mast cells
We found that forskolin (10−7 to 3×10−5M) caused dose-related inhibition of antigen-induced histamine release from human basophil leukocytes. The dose-response inhibition curve was paralleled by a forskolin-induced increase in cyclic AMP (cAMP) levels in human leukocyte preparations. The kinetics of inhibition of histamine release and of the increase in leukocyte cAMP were the same.
In a second series of experiments we evaluated the effect of forskolin on antigen-induced histamine release from chopped human lung passively sensitized with serum from an allergic patient. Forskolin (10−7 to 3×10−5M) dosedependently inhibited the release of histamine from human lung mast cells. Thus forskolin appears to modulate the release of mediators of the immediate hypersensitivity reaction, presumably through activation of adenylate cyclase in human basophils and mast cells.
KeywordsMast Cell Histamine Human Lung Hypersensitivity Reaction Adenylate Cyclase
Unable to display preview. Download preview PDF.
- M. Ricci andG. Marone,Progress in Clinical Immunology. The Role of Chemical Mediators and Cellular Interactions, Karger, Basel, 1983.Google Scholar
- G. Marone,The role of basophils and mast cells in the pathogenesis of pulmonary diseases, Int. Archs. Allergy Appl. Immun.76, suppl. 1, 70–82 (1985).Google Scholar
- G. Marone, G. Ambrosio, D. Bonaduce, A. Genovese, M. Triggiani andM. Condorelli,Inhibition of IgE-mediated histamine release from human basophils and mast cells by fenoterol, Int. Archs. Allergy Appl. Immun.74, 356–361 (1984).Google Scholar
- W. Kreutner, R. Chapman, B.A. Gulbenkian andS. Tozzi,Bronchodilator and anti-allergy activity of forskolin (abstr.), J. Allergy Clin. Immun.73, 130 (1984).Google Scholar
- B.J. Undem andC.K. Buckner,Forskolin inhibits antigen-induced histamine release from guinea-pig lung (abstr.), Fed. Proc.43, 369 (1984).Google Scholar