Agents and Actions

, Volume 11, Issue 4, pp 307–311 | Cite as

Influence of dietary histidine on tissue histamine concentration, histidine decarboxylase and histamine methyltransferase activity in the rat

  • Nam Soo Lee
  • Dennis Fitzpatrick
  • Eileen Meier
  • Hans Fisher
Histamine and Kinins


Three-week-old male rats were fed for two weeks diets supplying inadequate, adequate, or excess amounts of histidine. After the 2-week feeding of the experimental diets, the rats were killed. Brain, gastrocnemius muscle, kidney and stomach were removed and analyzed for histamine and free-histidine as well as for the degradative enzyme, HMT, and the histamine-synthesizing enzyme HDC.

The following results were obtained: As the level of dietary histidine increased, (1) tissue concentrations of free-histidine and of histamine increased in all the tissues analyzed. (2) The increase of histamine was greatest in brain and stomach (5- and 4-fold, respectively), but less in kidney and muscle (2-fold). (3) HDC activity was not detected in muscle, but doubled from the lowest to the highest histidine intake in brain and increased almost 6-fold between the lowest and the highest histidine levels in stomach. (4) Kidney HDC decreased from the lowest to the two higher levels of dietary histidine. (5) HMT activity increased nominally in brain and not significantly in kidney; none was detected in either muscle or stomach. (6) Brain and kidney, tissues with considerable HMT activity, had almost no histamine. The increases in tissue histamine concentrations observed in the tissues analyzed generally reflected the changes and magnitudes of enzyme activities for HMT and HDC. The results in the rat differ in important ways from those previously observed in chickens as follows: (1) Histamine concentrations as a function of dietary histidine decreased in the chick. (2) Both HDC and HMT activities were present in chick muscle tissue. (3) HDC activity in chick stomach decreased sharply as a function of dietary histidine.


Histamine Histidine Tissue Concentration Experimental Diet Gastrocnemius Muscle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. [1]
    M.R. Quinn andH. Fisher,Effect of Dietary Histidine on Olfaction, and Rat Brain and Muscle Concentration of Histidine-Containing Dipeptides, J. Neurochem.29, 717–728 (1977).Google Scholar
  2. [2]
    J.F. Amend, D. Strumeyer andH. Fisher,Effect of Dietary Histidine on Tissue Concentrations of Histidine-Containing Dipeptides in Adult Cockerels, J. Nutr.109, 1779–1786 (1979).Google Scholar
  3. [3]
    D.E. Fisher, J.F. Amend, D. Strumeyer andH. Fisher,A Role for Carnosine and Anserine in Histamine Metabolism of the Traumatized Rat, Proc. Soc. exp. Biol. Med.158, 402–405 (1978).Google Scholar
  4. [4]
    T. Ishibashi, O. Donis, D. Fitzpatrick, N.S. Lee, O. Turetsky andH. Fisher,Effect of Age and Dietary Histidine on Histamine Metabolism of the Growing Chick, Agents and Actions9, 435–444 (1979).Google Scholar
  5. [5]
    W. Lorenz, E. Matejka, A. Schmal, W. Seidel, H. Reimann, R. Uhlig andG. Mann,A Phylogenetic Study on the Occurrence and Distribution of Histamine in the Gastro-Intestinal Tract and Other Tissues of Man and Various Animals, Comp. Gen. Pharmac.4, 229–250 (1973).Google Scholar
  6. [6]
    W. Lorenz, H. Barth andE. Werle,Histamine and Histamine Methyltransferase in the Gastric Mucosa of Man, Pig, Dog and Cow, Naunyn-Schmiedebergs Arch. exp. Path. Pharmak.267, 421–432 (1970).Google Scholar
  7. [7]
    R.J. Taylor, Jr,Inhibition of Gastric Acid Secretion by 4-Imidazolyl-3-amino-2-butanone (McN-A-1293), a Specific Inhibitor of Histidine Decarboxylase, Biochem. Pharmac.27, 2653–2654 (1978).Google Scholar
  8. [8]
    H. Barth, W. Lorenz andH. Troidl,Effect of Amodiaquine on Gastric Histamine Methyltransferase and on Histamine-Stimulated Gastric Secretion, Br. J. Pharmac.55, 321–327 (1975).Google Scholar
  9. [9]
    D.S. Duch, S. Bowers, M. Edelstein andC.A. Nichol,Histamine: Elevation of Brain Levels by Inhibition of Histamine N-Methyl Transferase, Transmethylation (Elsevier North Holland, Inc., Amsterdam 1979), pp. 287–295.Google Scholar
  10. [10]
    T. Ishibashi, O. Donis, D. Fitzpatrick, N.S. Lee andH. Fisher,Histamine Synthesis and Degradation in the Chick (Gallus gallus) and the Rat (Rattus rattus), Comp. Biochem. Physiol.64C, 227–228 (1979).Google Scholar
  11. [11]
    R.E. Shaff andM.A. Beaven,Increased Sensitivity of the Enzymatic Isotopic Assay of Histamine: Measurement of Histamine in Plasma and Serum, Anal. Biochem.94, 425–430 (1979).Google Scholar
  12. [12]
    Q.R. Rogers andA.E. Harper,Amino Acid Diets and Maximal Growth in the Rat, J. Nutr.87, 267–273 (1965).Google Scholar
  13. [13]
    D. Barbaro, D.E. Fisher, D. Strumeyer andH. Fisher,Developmental Changes and Dietary Histidine Manipulation: Effect on Rat Olfactory Bulb and Leg Muscle Components, J. Nutr.108, 1348–1354 (1978).Google Scholar
  14. [14]
    Z. Huszti, A. Kenessey, M. Kurti andT.L. Sourkes,Non-Mast Cell Histamine Levels in Rat Tissues After Histidine Loading, Eur. J. Pharmac.42, 231–240 (1977).Google Scholar

Copyright information

© Birkhäuser Verlag 1981

Authors and Affiliations

  • Nam Soo Lee
    • 1
  • Dennis Fitzpatrick
    • 1
  • Eileen Meier
    • 1
  • Hans Fisher
    • 1
  1. 1.Department of NutritionRutgers UniversityNew BrunswickUSA

Personalised recommendations