Pharmaceutisch Weekblad

, Volume 14, Issue 2, pp 46–49 | Cite as

Liquid chromatographic estimation of tranylcypromine in human plasma

  • P. G. L. C. Krugers Dagneaux
  • C. P. G. G. Loohuis
  • J. T. Klein Elhorst
  • T. S. Van der Veer


A simple, selective and quantitative estimation of tranylcypromine using liquid chromatography and electrochemical detection with apomorphine as internal standard in human plasma is described. Sample preparation is done by liquid-liquid extraction. The absolute mean recovery of apomorphine is 95%, the recovery of tranylcypromine is 81%. This method gives a simple estimation method to assay plasma concentrations of tranylcypromine in the therapeutic range (5–200 ng/ml) with good reproducibility and linearity.


Chromatography, high pressure liquid Detection, electrochemical Plasma Tranylcypromine 


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  1. 1.
    Nolen WA. Komen de monoamine-oxidase-remmers weer terug? [Will there be a place again for monoamine oxidase inhibitors?]. Ned Tijdschr Geneeskd 1985; 129:1571–6.PubMedGoogle Scholar
  2. 2.
    Pare CMB. The present status of monoamine oxidase inhibitors. Br J Psychiatry 1985;146:576–84.PubMedGoogle Scholar
  3. 3.
    Brown CS, Bryant SG. Monoamine oxidase inhibitors: safety and efficacy issues. Drug Intell Clin Pharm 1988;22:232–5.PubMedGoogle Scholar
  4. 4.
    Lippman SB, Nash K. Monoamine oxidase inhibitor update. Potential adverse food and drug interactions. Drug Safety 1990;5:195–204.PubMedGoogle Scholar
  5. 5.
    Mallinger AG, Edwards DJ, Himmelhoch JM, Knop S, Ehler J. Pharmacokinetics of tranylcypromine in patients who are depressed: relationship to cardiovascular effects. Clin Pharmacol Ther 1986;40:444–50.PubMedGoogle Scholar
  6. 6.
    Mallinger AG, Himmelhoch JM, Thase ME, Edwards DJ, Knop S. Plasma tranylcypromine: relationship to pharmacokinetic variables and clinical antidepressant actions. J Clin Psychopharmacol 1990;10:176–83.PubMedGoogle Scholar
  7. 7.
    Baselt RC, Stewart CB, Shaskan E. Determination of serum and urine concentrations of tranylcypromine by electron-capture gas-liquid chromatography. J Anal Toxicol 1977;1:215–7.Google Scholar
  8. 8.
    Baily E, Barron EJ. Determination of tranylcypromine in human plasma and urine using high resolution gas-liquid chromatography with nitrogen-sensitive detection. J Chromatogr/Biomed Applic 1980;183:25–31.Google Scholar
  9. 9.
    Edwards DJ, Mallinger AG, Knopf S, Himmelhoch JM. Determination of tranylcypromine in plasma using gas chromatography—chemical-ionisation mass spectrometry. J Chromatogr/Biomed Applic 1985;344:356–61.Google Scholar
  10. 10.
    Weber H, Spahn H, Mutschier E. Activated alphaalkyl alpha-arylacetic acid enantiomers for stereoselective thin layer chromatographic and high-performance liquid chromatographic determination of chiral amines. J Chromatogr/Biomed Applic 1984;307: 145–53.Google Scholar
  11. 11.
    Essink AWG, Loohuis CP, Klein Elhorst JT, Rutten WJ. Selective and quantitative isolation and determination of apomorphine in human plasma. J Chromatogr/Biomed Applic 1991;170:419–24.CrossRefGoogle Scholar
  12. 12.
    Smedes P, Kraak JS, Poppe H. Simple and fast solvent extraction system for selective and quantitative isolation of adrenaline, noradrenaline and dopamine from plasma and urine. J Chromatogr/Biomed Applic 1982; 231:25–39.Google Scholar
  13. 13.
    Lie-A-Huen L, Stuurman RM, Ijdenberg FN, Kingma JH, Meijer DK. High-performance liquid Chromatographic assay of flecainide and its enantiomers in serum. Ther Drug Monitor 1989;11:708–11.Google Scholar

Copyright information

© Royal Dutch Association for Advancement of Pharmacy 1992

Authors and Affiliations

  • P. G. L. C. Krugers Dagneaux
    • 1
  • C. P. G. G. Loohuis
    • 2
  • J. T. Klein Elhorst
    • 2
  • T. S. Van der Veer
    • 2
  1. 1.Department of Clinical PharmacyMedisch Spectrum TwenteKA Enschedethe Netherlands
  2. 2.Department of PsychiatryMedisch Spectrum Twentethe Netherlands

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