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Netherlands Journal of Plant Pathology

, Volume 74, Supplement 1, pp 67–80 | Cite as

The enzymatic maceration of plant tissue

  • D. F. Bateman
Article

Abstract

A new technique was developed for measuring the enzymatic maceration of plant tissue. When potato tissue disks were subjected to constant agitation in the presence of a “macerating enzyme” they were reduced to a suspension of single cells. the maceration process was followed by determining the increase in turbidity of reaction mixtures at 475 mμ due to the release of free cells. The process of tissue maceration as measured by this turbidimetric procedure was observed to consist of a lag phase followed by a linear phase of cell release; the length of the lag phase and the slope of the linear phase of cell release were both related to the log of the “macerating enzyme” concentration. The “macerating enzyme” ofSclerotium rolfsii was purified and identified as an endo-polygalacturonase.

Samenvatting

Een nieuwe methode werd ontwikkeld om de enzymatische maceratie van planteweefsel te meten. Schijfjes aardappel werden in een oplossing van een macererend enzym in voortdurende beweging gehouden, waardoor een suspensie van afzonderlijke cellen ontstond. Het verloop van de maceratie werd vervolgd door de toename der troebeling bij 475 mμ. Het maceratieproces, gemeten volgens deze turbidimetrische methode, bleek eerst een “lag phase” te vertonen, daarna verliep het lineair met de tijd. De lengte van de “lag phase” en de hellingshoek van de lineaire fase hielden beide verband met de concentratie van het macererende enzym.

Het macererende enzym vanSclerotium rolfsii is gezuiverd en geïdentificeerd als een endo-polygalacturonase.

Keywords

Enzyme Plant Pathology Turbidity Single Cell Plant Tissue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 1968

Authors and Affiliations

  • D. F. Bateman
    • 1
  1. 1.Department of Plant PathologyCornell UniversityIthacaU.S.A.

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