Transgenic Research

, Volume 2, Issue 4, pp 208–218

NewAgrobacterium helper plasmids for gene transfer to plants

  • Elizabeth E. Hood
  • Stanton B. Gelvin
  • Leo S. Melchers
  • Andre Hoekema
Papers

Abstract

We describe the construction of new helper Ti plasmids forAgrobacterium-mediated plant transformation. These plasmids are derived from three differentAgrobacterium tumefaciens Ti plasmids, the octopine plasmid pTiB6, the nopaline plasmid pTiC58, and the L,L-succinamopine plasmid pTiBo542. The T-DNA regions of these plasmids were deleted using site-directed mutagenesis to yield replicons carrying thevir genes that will complement binary vectorsin trans. Data are included that demonstrate strain utility. The advantages ofAgrobacterium strains harbouring these ‘disamed’ Ti plasmids for plant transformation viaAgrobacterium are discussed.

Keywords

Agrobacterium plant transformation helper plasmids host range Ti plasmid T-DNA 

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Copyright information

© Chapman & Hall 1993

Authors and Affiliations

  • Elizabeth E. Hood
    • 1
  • Stanton B. Gelvin
    • 2
  • Leo S. Melchers
    • 3
  • Andre Hoekema
    • 3
  1. 1.Department of BiologyUtah State UniversityLoganUSA
  2. 2.Department of Biological SciencesPurdue UniversityWest LafayetteUSA
  3. 3.MOGEN InternationalLeidenNetherlands

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