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Transgenic Research

, Volume 4, Issue 1, pp 18–25 | Cite as

Expression of snowdrop lectin in transgenic tobacco plants results in added protection against aphids

  • V. A. Hilder
  • K. S. Powell
  • A. M. R. Gatehouse
  • J. A. Gatehouse
  • L. N. Gatehouse
  • Y. Shi
  • W. D. O. Hamilton
  • A. Merryweather
  • C. A. Newell
  • J. C. Timans
  • W. J. Peumans
  • E. van Damme
  • D. Boulter
Papers

Abstract

The range of sap-sucking insect pests to which GNA, (the mannose specific lectin from snowdrops (Galanthus nivalis) has been shown to be insecticidal in artificial diets has been extended to include the peach potato aphid (Myzus persicae). A gene construct for constitutive expression of GNA from the CaMV35S gene promoter has been introduced into tobacco plants. A transgenic tobacco line which expresses high levels of GNA has been shown to have enhanced resistance toM. persicae in leaf disc and whole plant bioassays,demonstrating the potential for extending transgenic plant technology to the control of sap-sucking insect pests.

Keywords

transgenic crops insect resistance aphid homoptera lectin crop protection Nicotiana tabacum 

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Copyright information

© Chapman & Hall 1995

Authors and Affiliations

  • V. A. Hilder
    • 1
  • K. S. Powell
    • 1
  • A. M. R. Gatehouse
    • 1
  • J. A. Gatehouse
    • 1
  • L. N. Gatehouse
    • 1
  • Y. Shi
    • 1
  • W. D. O. Hamilton
    • 2
  • A. Merryweather
    • 2
  • C. A. Newell
    • 2
  • J. C. Timans
    • 2
  • W. J. Peumans
    • 3
  • E. van Damme
    • 3
  • D. Boulter
    • 1
  1. 1.Department of Biological SciencesUniversity of DurhamDurhamUK
  2. 2.AXIS Genetics LtdCambridgeUK
  3. 3.Laboratory for Phytopathology and Plant ProtectionKatholieke Universiteit LeuvenHeverleeBelgium

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