Experientia

, Volume 36, Issue 10, pp 1141–1143 | Cite as

Quantification of digoxin by enzyme immunoassay: synthesis of a maleimide derivative of digoxigenin succinate for enzyme coupling

  • N. Monji
  • H. Ali
  • A. Castro
Specialia

Summary

We synthesized the m-maleimidobenzoyl derivative of digoxigenin-3-0-succinate (through a p-phenylenediamine bridge) as a hapten derivative directed towards coupling to sulfhydryl groups of β-galactosidase. Prepared enzyme conjugate had about 97% of the enzyme labeled with the hapten derivative while retaining full enzyme activity. The enzyme immunoassay for digoxin we prepared showed a maximum sensitivity of 30 pg per assay (c.v.=3%) with minimal cross-reaction with digotoxin (3.8%). Our method for hapten conjugation to β-galactosidase is highly efficient and is simple and easily replicated.

Keywords

Enzyme Enzyme Activity Succinate Digoxin Enzyme Immunoassay 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Birkhäuser Verlag 1980

Authors and Affiliations

  • N. Monji
    • 1
  • H. Ali
    • 1
  • A. Castro
    • 1
  1. 1.Hormone Research Laboratory, Department of PathologyUniversity of Miami School of MedicineMiamiUSA

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