, Volume 42, Issue 1, pp 23–30 | Cite as

Comparative cytochemical and pharmacological studies on the cholinergic innervation of the branchial heart of the cephalopodSepia officinalis (L.)

  • R. Schipp
  • H. R. Schmidt
  • A. Fiedler
Full Papers


The dense motor innervation of the obliquely striated muscle cells in the branchial heart of Coleoida is composed of activating and inhibiting parts. The inhibiting cholinergic system investigated in this study is characterized histo- and cytochemically by a high acetylcholinesterase activity (EC in the glycocalices of the nerve fibers with transparent synaptic vesicles, the muscle cells and, to a lesser extent, the ovoid interstitial cells, the functions of which are endocytosis and storage of catabolic substances. The pharmacological results from the isolated organ indicate a more nicotinic type of Ach-receptor, which can be reversibly blocked by D-tubocurarine and α-bungarotoxin, but not with the same intensity by tetraethylammonium or atropine.

Key words

Branchial heart cephalopods cholinergic innervation 


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  1. 1.
    Alexandrowicz, J.S., Innervation of the hearts ofSepia officinalis. Acta zool. Stockh.41 (1960) 65–100.Google Scholar
  2. 2.
    Bacq, Z.M., Réactions du ventricule médian isolé deLoligo pealii à l'acétylcholin, à l'atropine et aux ions K, Ca et Mg. C. r. Soc. Biol.114 (1933) 1360–1361.Google Scholar
  3. 3.
    Bodian, D., The staining of paraffin sections of nervous tissues with activated protargol. Anat. Rec.69 (1937) 153–162.CrossRefGoogle Scholar
  4. 4.
    Chang, C.C., and Lee, C.J., Isolation of neurotoxins from the venom ofBungarus multicinctus and their modes of neuromuscular blocking action. Archs int. Pharmacodyn.144 (1963) 241–257.Google Scholar
  5. 5.
    Fumagalli, L., De-Renzis, G., and Miani, N., Acetylcholine receptors: number and distribution in intact and deafferented superior cervical ganglion of the rat. J. Neurochem.27 (1976) 47–52.PubMedGoogle Scholar
  6. 6.
    Ghiretti, F., Action of choline and acetylcholine on the isolated heart of gastropod and cephalopod molluscs. Archs Sci. biol.32 (1948) 239–251.Google Scholar
  7. 7.
    Johansen, K., and Huston, M.J., Effects of some drugs on the circulation system of the intact, non-anesthetized cephalopodOctopus dofleini. Comp. Biochem. Physiol.5 (1962) 177–184.CrossRefGoogle Scholar
  8. 8.
    Karnovsky, M.J., and Roots, L., A ‘direct-coloring’ thiocholine method for choline-esterase. J. Histochem. Cytochem.12 (1964) 219–221.PubMedGoogle Scholar
  9. 9.
    Kasa, P., and Csillik, B., Electron microscopic localization of cholinesterase by a copper-lead-thiocholine technique. J. Neurochem.13 (1966) 1345–1349.PubMedGoogle Scholar
  10. 10.
    Katz, B., and Miledi, R., The effect of α-bungarotoxin on acetylcholine receptors. Br. J. Pharmac.49 (1973) 138–139.Google Scholar
  11. 11.
    Kehoe, J.S., Sealock, R., and Bon, C., Effects of α-toxins fromBungarus multicinctus andB. caeruleus on cholinergic responses inAplysia neurones. Brain Res.107 (1976) 527–540.CrossRefPubMedGoogle Scholar
  12. 12.
    Kling, G., Innervation und Pharmakologie des Zentralherzens vonSepia officinalis (L.). Verh. dt. zool. Ges.76 (1983) 298.Google Scholar
  13. 13.
    Kruta, V., Sur l'action de l'acetylcholine et de l'atropine sur la cœur deSepia officinalis. C. r. Soc. Biol.119 (1935) 608.Google Scholar
  14. 14.
    Leake, L.D., and Walker, R.J., Invertebrate neuropharmacology, p. 86. Blackie, Glasgow and London 1980.Google Scholar
  15. 15.
    Lee, C.Y., Tseng, L.F., and Chin, T.H., Influence of denervation in localization of neurotoxins from elapid venoms in rat diaphragm. Nature215 (1976) 1177–1178.Google Scholar
  16. 16.
    Mislin, H., and Kauffmann, M., Der aktive Gefässpuls in der Arm-Schirmhaut der Cephalopoden. Revue suisse Zool.55 (1948) 267–271.Google Scholar
  17. 17.
    Mislin, H., Über Beziehungen zwischen Atmung und Kreislauf bei Cephalopoden (Sepia officinalis L.). Synchronregistrierung von Elektrokardiogramm (EKG) und Atembewegung am schwimmenden Tier. Zool. Anz., Suppl.30 (1967) 175–181.Google Scholar
  18. 18a.
    Schipp, R., and Schäfer, A., Vergleichende elektronenmikroskopische Untersuchungen an den zentralen Herzorganen von Cephalopoden. Feinstruktur des Herzens. Z. Zellforsch.98 (1969) 576–698.CrossRefPubMedGoogle Scholar
  19. 18b.
    Schipp, R., and Schäfer, A., Vergleichende elektronenmikroskopische Untersuchungen an den zentralen Herzorganen von Cephalopoden. Feinstruktur und Funktion der Kiemenherzen. Z. Zellforsch.101 (1969) 367–379.CrossRefPubMedGoogle Scholar
  20. 19.
    Schipp, R., Schäfer, A., and Höhn, P., Elektronenmikroskopische und histochemische Untersuchungen zur Funktion des Kiemenherzanhanges (Pericardialdrüse) vonSepia officinalis. Z. Zellforsch.117 (1971) 252–274.CrossRefPubMedGoogle Scholar
  21. 20.
    Schipp, R., and Hevert, F., Distribution of copper and iron in some central organs ofSepia officinalis (Cephalopoda). A comparative study by flameless atomic absorption and electron microscopy. Marine Biol.47 (1978) 391–399.CrossRefGoogle Scholar
  22. 21.
    Schipp, R., and Hevert, F., Ultrafiltration in the branchial heart appendage of dibranchiate cephalopods: a comparative ultrastructural and physiological study. J. exp. Biol.92 (1981) 23–35.Google Scholar
  23. 22.
    Sundermann, G., Die Ultrastruktur der vakuolisierten Rundzellen vonLoligo vulgaris Lam. (Mollusca, Cephalopoda). Zool. Jb. Anat.103 (1980) 93–104.Google Scholar
  24. 23.
    Wells, M.J., and Mangold, K., The effects of extracts from neurosecretory cells in the anterior vena cava and pharyngoophthalmic vein upon the hearts of intact free-moving octopuses. J. exp. Biol.84 (1980) 319–334.Google Scholar
  25. 24.
    Wells, M.J., Nervous control of the heartbeat inOctopus. J. exp. Biol.85 (1980) 111–128.PubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag 1986

Authors and Affiliations

  • R. Schipp
    • 1
    • 2
  • H. R. Schmidt
    • 1
    • 2
  • A. Fiedler
    • 1
    • 2
  1. 1.Institut für Allgemeine und Spezielle ZoologieJustus Liebig UniversityGiessen(Federal Republic of Germany)
  2. 2.Station Biologique d'ArcachonArcachon(France)

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