Use of pooled residual laboratory sera to assess human immunodeficiency virus prevalence among patients in Italy

  • V. Puro
  • E. Lo Presti
  • R. Trombetta
  • A. Benedetto
  • G. P. Leonetti
  • A. Spano
  • G. Ippolito
  • the Italian Study Group on Occupational Risk of HIV Infection
Article

DOI: 10.1007/BF01974538

Cite this article as:
Puro, V., Lo Presti, E., Trombetta, R. et al. Eur. J. Clin. Microbiol. Infect. Dis. (1994) 13: 205. doi:10.1007/BF01974538

Abstract

An anonymous unlinked seroprevalence study of human immunodeficiency virus (HIV) infection was performed by testing pools of ten sera remaining from specimens submitted consecutively to clinical pathology laboratories at 18 Italian public hospitals during four consecutive days in April 1991. Sera from positive pools were retested individually by three different enzyme immunoassays (EIAs) and considered positive if reactive by all three assays. Only the sera with discordant EIA results were retested by Western blot. Of a total of 22,590 sera, 278 were HIV positive (1.2 %). The highest rates were seen in hospitals located in metropolitan areas (1.5 %), in infectious disease departments (28 %) and in drug addiction treatment units (28 %); among men aged 21–30 (4.6 %) and 31–40 years (4 %); and among women aged 21–30 years (1.6 %). The distribution of seropositive patients by gender and age group suggests an increasing role of heterosexual transmission of the infection. The presence of anti-HIV antibodies in sera from patients of both sexes, in all age groups, and from all clinical settings reinforces the need for health care workers to adhere to universal precautions issued to prevent occupational bloodborne infections.

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1994

Authors and Affiliations

  • V. Puro
    • 1
  • E. Lo Presti
    • 2
  • R. Trombetta
    • 2
  • A. Benedetto
    • 2
  • G. P. Leonetti
    • 3
  • A. Spano
    • 3
  • G. Ippolito
    • 1
  • the Italian Study Group on Occupational Risk of HIV Infection
  1. 1.Unita' Operativa AIDS RM-10Ospedale L. SpallanzaniRomeItaly
  2. 2.Laboratorio di VirologiaOspedale San CamilloRomeItaly
  3. 3.Laboratorio di Patologia Clinica e MicrobiologiaOspedale C. ForlaniniRomeItaly

Personalised recommendations