Archives of Toxicology

, Volume 66, Issue 8, pp 587–591 | Cite as

Effect of tetrakis-μ-3,5-diisopropylsalicylatodiaquodicopper(II) on the status of reduced glutathione in freshly isolated hepatocytes

  • Muhammad Farid Khan
  • Yasuo Ohno
  • Akira Takanaka
Original Investigations
Received:
Accepted:

Abstract

Effects of different concentrations of tetrakis-μ-3,5-diisopropylsalicylatodiaquodicopper(II) (Cu(II)2(3,5-DIPS)4(H2O)2) on the reduced status of glutathione (GSH), the major nonprotein thiol in tissues, were investigated using freshly isolated hepatocytes. Cu(II)2(3,5-DIPS)4 below 100 μM did not have any significant effects on either the GSH content or viability of the hepatocytes, but at 150–250 μM it decreased both parameters after 1 h of incubation. The decrease in cellular GSH was not followed by an increase in the oxidized form of GSH (GSSG) in the cell suspension. The addition of deferoxamine with Cu(II)2(3,5-DIPS)4 to the hepatocyte suspension prevented depletion in GSH content and loss of cell viability by Cu(II)2(3,5-DIPS)4. Both GSH depletion and loss of cell viability were found to be Cu(II)2(3,5-DIPS)4 dose dependent. From these results, it appears that Cu(II)2(3,5-DIPS)4 penetrated the cell membrane and acted by decreasing the GSH level by forming a copper-glutathione complex.

Key words

l-Glutathione Glutathione disulfide Copper(II) complex Cu(II)-SG complex Hepatocyte 
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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Muhammad Farid Khan
    • 1
  • Yasuo Ohno
    • 1
  • Akira Takanaka
    • 1
  1. 1.Division of Pharmacology, Biological Safety Research CenterNational Institute of Hygienic SciencesTokyoJapan

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