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Archives of Toxicology

, Volume 64, Issue 7, pp 554–561 | Cite as

Detection of anticonvulsants and their metabolites in urine within a “general unknown” analysis procedure using computerized gas chromatography-mass spectrometry

  • Hans H. Maurer
Article

Abstract

Detection of the anticonvulsants carbamazepine, clonazepam, diazepam, ethosuximide, mephenytoin, mesuximide, methylphenobarbital, phenobarbital, phenytoin, primidone, propylhexedrine, sultiame, trimethadion and their metabolites in urine is described. The method presented is integrated in a general screening procedure (general unknown analysis) for several groups of drugs, detecting several hundred drugs and over 1000 metabolites. It includes cleavage of conjugates by acid hydrolysis, isolation by liquid-liquid extraction, derivatization by acetylation, separation by capillary gas chromatography and identification by computerized mass spectrometry. Using mass chromatography with the selective ions m/z 58, 104, 113, 117, 165, 193, 204 and 246, the possible presence of anticonvulsants and/or their metabolites was indicated. The identity of positive signals in the reconstructed mass chromatograms was confirmed by a visual or computerized comparison of the stored full mass spectra with the reference spectra. The sample preparation, mass chromatograms, reference mass spectra and gas chromatographic retention indices are documented.

Key words

Detection Anticonvulsant Metabolites Gas chromatography-mass spectrometry Analytical toxicology 

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Hans H. Maurer
    • 1
  1. 1.Institut für Pharmakologie und Toxikologie der Universität des SaarlandesHomburgGermany

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