Archives of Toxicology

, Volume 67, Issue 7, pp 497–501 | Cite as

Possible involvement of active oxygen species in selenite toxicity in isolated rat hepatocytes

  • Jun Kitahara
  • Yoshiyuki Seko
  • Nobumasa Imura
Original Investigations

Abstract

Mechanisms of selenite cytotoxicity were examined using isolated rat hepatocytes. When selenite was added to a suspension of rat hepatocytes, intracellular reduced glutathione (GSH) was decreased and the oxygen consumption rate was increased. Subsequently, thiobarbituric acid-reactive substances (TBA-RS) and lactate dehydrogenase (LDH) leakage were increased. A ferric iron chelator, desferrioxamine (DF), and a synthetic Superoxide dismutase (SOD) mimic, desferrioxamine manganese (DFMn), reduced the selenite toxicity. These results suggest that Superoxide anion and its reactive metabolites such as the hydroxyl radical may be involved in the cytotoxicity of selenite.

Key words

Selenite toxicity Superoxide anion Active oxygen Rat hepatocytes 

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Jun Kitahara
    • 1
  • Yoshiyuki Seko
    • 1
  • Nobumasa Imura
    • 1
  1. 1.Department of Public Health, School of Pharmaceutical SciencesKitasato UniversityTokyoJapan

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