Transgenic Research

, Volume 2, Issue 2, pp 115–120

The use of the nonradioactive digoxigenin chemiluminescent technology for plant genomic Southern blot hybridization: A comparison with radioactivity

  • Gabriele Neuhaus-Url
  • Gunther Neuhaus
Article

Abstract

A nonradioactive labelling and detection method for plant genomic DNA analysis is compared to the radioactive method. The radioisotopes are replaced by a nucleotide, digoxigenin-11-dUTP, and the signal detection is accomplished by the enzymatic reaction of alkaline phosphatase, conjugated to anti-digoxigenin antibodies, with the chemiluminescent substrate AMPPD (3-(2′-spiroadamantane)-4-methoxy-4(3″ phosphorytoxy) phenyl-1, 2-dioxetane). The sensitivity of the radioactive and nonradioactive methods are directly compared using identical Southern blots subjected to the radioactive and nonradioactive detection. The advantages of this nonradioactive method are discussed.

Keywords

chemiluminescence digoxigenin nonradioactive plant DNA Southern blot hybridization 

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Copyright information

© Chapman & Hall 1993

Authors and Affiliations

  • Gabriele Neuhaus-Url
    • 1
  • Gunther Neuhaus
    • 1
  1. 1.Institute for Plant SciencesSwiss Federal Institute of Technology ZürichZürichSwitzerland

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