Transgenic Research

, Volume 4, Issue 4, pp 288–290 | Cite as

pBINPLUS: An improved plant transformation vector based on pBIN19

  • Fred A. van Engelen
  • Jos W. Molthoff
  • Anthony J. Conner
  • Jan-Peter Nap
  • Andy Pereira
  • Willem J. Stiekema
Short Communication

Abstract

We describe the construction of a new plant transformation vector, pBINPLUS, based on the popular pBIN19 vector. Improvements over pBIN19 include location of the selectable marker gene at the left T-DNA border, a higher copy number inE. coli, and two rare restriction sites around the multiple cloning site for easier cloning and analysis of T-DNA insertions in plant genomes.

Keywords

transformation vector pBIN19 transgenic plants T-DNA Agrobacterium tumefaciens 

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References

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Copyright information

© Chapman & Hall 1995

Authors and Affiliations

  • Fred A. van Engelen
    • 1
  • Jos W. Molthoff
    • 1
  • Anthony J. Conner
    • 1
  • Jan-Peter Nap
    • 1
  • Andy Pereira
    • 1
  • Willem J. Stiekema
    • 1
  1. 1.Department of Molecular BiologyCentre for Plant Breeding and Reproduction Research (CPRO-DLO)WageningenThe Netherlands

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