Detection of extended broad-spectrum beta-lactamases inEnterobacteriaceae in four French hospitals
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Abstract
In 210 strains ofEnterobacteriaceae which were isolated in four hospitals and which showed reduced susceptibility to cefotaxime, high synergy was demonstrated between amoxicillin (20µg) + clavulanate (10µg) and cefotaxime (30µg) using a simple double-disk test. Isoelectric focusing on gel and specific iodometric detection using ceftriaxone identified four extended broad-spectrumβ-lactamases (isoelectric points 7.6, 6.3, 7.0 and 5.9) produced by the strains.
Keywords
Internal Medicine Amoxicillin Ceftriaxone Isoelectric Point Cefotaxime
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