Western blot analysis of sera from Lyme borreliosis patients according to the genomic species of theBorrelia strains used as antigens

  • M. V. Assous
  • D. Postic
  • G. Paul
  • P. Névot
  • G. Baranton


Sera of 52 Lyme borreliosis patients classified according to their clinical features were analysed by Western blot using as antigensBorrelia strains belonging to three recently described genomic species. The antibody response was demonstrated to be homologous within each genospecies. Serum reactivity was studied for each of the type strainsBorrelia burgdorferi sensu stricto (strain B31T),Borrelia garinii (strain 20047T) and group VS461. Seven of 15 sera (46.6 %) of patients with menin-goradiculitis showed preferential reactivity withBorrelia garinii (strain 20047T), all of 8 sera (100 %) of patients with acrodermatitis chronica atrophicans showed preferential reactivity with group VS461 (strain VS461) and 8 of 16 sera (50 %) of patients with arthritis showed preferential reactivity withBorrelia burgdorferi sensu stricto (strain B31T). The presence of a strong response to OspA and OspB proteins ofBorrelia burgdorferi sensu stricto was found only in this last group of patients. These results suggest that there are clinical implications of the recently described modifications in the taxonomy ofBorrelia burgdorferi.


Arthritis Western Blot Blot Analysis Western Blot Analysis Antibody Response 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Burgdorfer W, Barbour AG, Hayes SF, Benach JL, Grundwald E, Davis JP: Lyme disease: a tick-borne spirochetosis? Science 1982, 216: 1317–1319.PubMedGoogle Scholar
  2. 2.
    Steere AC, Grodzicki RL, Kornblatt AN, Craft JE, Barbour AG, Burgdorfer W, Schmid GP, Johnson E, Malawista SE: The spirochetal etiology of Lyme disease. New England Journal of Medicine 1983, 308: 733–740.PubMedGoogle Scholar
  3. 3.
    Steere AC: Lyme disease. The new England Journal of Medicine 1989, 321: 586–596.PubMedGoogle Scholar
  4. 4.
    Ackermann R, Hörtrup P, Schmidt R: Tick-borne meningopolyneuritis (Grain-Bujadoux, Bannwarth). Yale Journal of Biology and Medicine 1984, 57: 485–490.PubMedGoogle Scholar
  5. 5.
    Weber K, Schierz G, Wilske B, Preac-Mursic V: European erythema migrans disease and related disorders. Yale Journal of Biology and Medicine 1984, 57: 463–473.PubMedGoogle Scholar
  6. 6.
    Asbrink E, Hovmark A: Successful cultivation of spirochetes from skin lesions of patients with erythema chronicum migrans Afzelius and acrodermatitis chronica atrophicans. Acta Pathologica Microbiologica Immunologica Scandinavica [B] 1985, 93: 161–163.Google Scholar
  7. 7.
    Barbour AG, Heiland RA, Howe TR: Heterogeneity of major proteins in Lyme disease borreliae: a molecular analysis of North American and European isolates. Journal of Infectious Diseases 1985, 152: 478–484.PubMedGoogle Scholar
  8. 8.
    Wilske B, Preac-Mursic V, Schierz G: Antigenic heterogeneity of EuropeanBorrelia burgdorferi strains isolated from patients and ticks. Lancet 1985, i: 2099.Google Scholar
  9. 9.
    Wilske B, Preac-Mursic V, Schierz G, Busch KV: Immunochemical and immunological analysis of EuropeanBorrelia burgdorferi strains. Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene (A) 1986, 263: 92–102.Google Scholar
  10. 10.
    Barbour A: Classification ofBorrelia burgdorferi on the basis of plasmid profiles. Zentralblatt für Bakteriologie 1989, Supplement 18: 1–7.Google Scholar
  11. 11.
    Wilske B, Preac-Mursic V, Schierz G, Kühbeck R, Barbour A, Kramer M: Antigenic variability ofBorrelia burgdorferi. Annals of the New York Academy of Sciences 1988, 539: 126–143.PubMedGoogle Scholar
  12. 12.
    Postic D, Edlinger C, Richaud C, Grimont F, Dufresne Y, Perolat P, Baranton G, Grimont PAD: Two genomic species inBorrelia burgdorferi. Research in Microbiology 1990, 141: 465–475.CrossRefPubMedGoogle Scholar
  13. 13.
    Baranton G, Postic D, Saint Girons I, Boerlin P, Piffaretti JC, Assous M, Grimont PAD: Delineation ofBorrelia burgdorferi sensu stricto,Borrelia garinii sp. nov., and group VS461 associated with Lyme borreliosis. International Journal of Systematic Bacteriology 1992, 42: 378–383.PubMedGoogle Scholar
  14. 14.
    Wilske B, Schierz G, Preac-Mursic V, Weber K, Pfister HW, Einhaupl K: Serological diagnosis of erythema migrans disease and related disorders. Infection 1984, 12: 331–337.CrossRefPubMedGoogle Scholar
  15. 15.
    Assous M: A propos du diagnostic biologique de la borréliose de Lyme. Médecine et Maladies Infectieuses 1989, 19: 734–740.CrossRefGoogle Scholar
  16. 16.
    Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227: 680–85.PubMedGoogle Scholar
  17. 17.
    Marconi TR, Garon CF: Phylogenetic analysis of the genusBorrelia: a comparison of North American and European isolates ofBorrelia burgdorferi. Journal of Bacteriology 1992, 174: 241–244.PubMedGoogle Scholar
  18. 18.
    Picken RN: Polymerase chain reaction primers and probes derived from flagellin gene sequences for specific detection of the agents of Lyme disease and North American relapsing fever. Journal of Clinical Microbiology 1992, 30: 99–114.PubMedGoogle Scholar
  19. 19.
    Rosa PA, Hogan D, Schwan TG: Polymerase chain reaction analyses two distinct classes ofBorrelia burgdorferi. Journal of Clinical Microbiology 1991, 29: 524–532.PubMedGoogle Scholar
  20. 20.
    Welsh J, Pretzman C, Postic D, Saint Girons I, Baranton G, McClelland M: Genomic fingerprinting by arbitrarily primed polymerase chain reaction resolvesBorrelia burgdorferi into three distinct phyletic groups. International Journal of Systematic Bacteriology 1992, 42: 370–377.PubMedGoogle Scholar
  21. 21.
    Boerlin P, Peter O, Bretz AG, Postic D, Baranton G, Piffaretti JC: Population genetic analysis ofBorrelia burgdorferi isolates by multilocus enzyme electrophoresis. Infection and Immunity 1992, 60: 1677–1683.PubMedGoogle Scholar
  22. 22.
    Adam T, Gassmann GS, Rasiah C, Göbel UB: Phenotypic and genotypic analysis ofBorrelia burgdorferi isolates from various sources. Infection and Immunity 1991, 59: 2579–2585.PubMedGoogle Scholar
  23. 23.
    Peter O, Bretz AG: Polymorphism of outer surface proteins ofBorrelia burgdorferi as a tool for the classification. Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene 1992, 277: 28–33.Google Scholar
  24. 24.
    Zöller L, Burkgard S, Schäfer H: Validity of Western immunoblot band patterns in the serodiagnosis of Lyme borreliosis. Journal of Clinical Microbiology 1991, 29: 174–182.PubMedGoogle Scholar
  25. 25.
    Wilske B, Preac-Mursic V, Schierz G, Liegl G, Gueye W: Detection of IgM and IgG antibodies toBorrelia burgdorferi using different strains as antigen. Zentralblatt für Bakteriologie 1989, Supplement i8: 299–309.Google Scholar
  26. 26.
    Wilske B, Schierz G, Preac-Mursic V, Von Busch K, Kuhbeck R, Pfister HW, Einhaupl K: Intrathecal production of specific antibodies againstBorrelia burgdorferi in patients with lymphocytic meningoradiculitis (Bannwarth's syndrome). Journal of Infectious Diseases 1986, 153: 304–314.PubMedGoogle Scholar
  27. 27.
    Wilske B, Preac-Mursic V, Schierz G, Gueye W, Herzer P, Weber K: Immunochemische Analyse der Immunantwort bei spätmanifestationen des Lyme Borreliose. Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene 1988, A 267: 549–558.Google Scholar
  28. 28.
    Karlsson M, Hovind-Hougen K, Svenungsson B, Stiernstedt G: Cultivation and characterization of spirochetes from cerebrospinal fluid of patients with Lyme borreliosis. Journal of Clinical Microbiology 1990, 28: 473–479.PubMedGoogle Scholar
  29. 29.
    Karlsson M: Antibody response against autologous and heterologous isolates ofBorrelia burgdorferi in four patients with Lyme neuroborreliosis. European Journal of Clinical Microbiology & Infectious Diseases 1991, 10: 742–745.Google Scholar
  30. 30.
    Ciesielski CA, Markowitz LE, Horsley R, Hightower AW, Russel H, Broome CV: Lyme disease surveillance in the United States, 1983–1986. Reviews of Infectious Diseases 1989, 11, Supplement 6: 1435–1441.Google Scholar
  31. 31.
    Buchwald A: Ein Fall von diffuser idiopathischer Hautatrophie. Archives of Dermatology and Syphilology 1883, 110: 553–556.Google Scholar
  32. 32.
    Afzelius A: Verhandlungen der dermatologischen Gesellschaft zu Stockholm. Archives of Dermatology and Syphilology 1910, 110: 404.Google Scholar
  33. 33.
    Garin C, Bujadoux. Paralysie par les tiques. Journal de Médecine de Lyon 1922, 3: 765–767.Google Scholar
  34. 34.
    Bannwarth A: Chronische lymphozytäre Meningitis, entzündliche Polyneuritis and “Rheumatismus”. Archiv für Psychiatrie und Nervenkrankheiten 1941, 113: 284–376.Google Scholar
  35. 35.
    Steere AC, Malawista SE, Snydman DR, Shope RE, Andiman WA, Ross MR, Steele FM: Lyme arthritis: an epidemic of oligoarticular arthritis in children and adults in three Connecticut communities. Arthritis and Rheumatism 1977, 20: 7–17.PubMedGoogle Scholar
  36. 36.
    Anderson JF, Doby JM, Courtarmanach A, Hyde FW, Johnson RC: Différences antigéniques entre des souches deBorrelia burgdorferi isolées d'Ixodes ricinus en Bretagne. Médecine et Maladies Infectieuses 1986, 3: 171–175.CrossRefGoogle Scholar
  37. 37.
    Allal J, Thomas P, Vievres C, Mazzonelli J, Baranton G: La maladie de Lyme — isolement d'uneBorrelia du liquide céphalo-rachidien. Médecine et Maladies Infectieuses 1986, 6: 445–446.CrossRefGoogle Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1993

Authors and Affiliations

  • M. V. Assous
    • 1
  • D. Postic
    • 2
  • G. Paul
    • 1
  • P. Névot
    • 1
  • G. Baranton
    • 2
  1. 1.Laboratoire de BactériologieFaculté de Médecine CochinParisFrance
  2. 2.Unité de Bactériologie Moléculaire et MédicaleInstitut PasteurParis Cedex 15France

Personalised recommendations