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Western blot analysis of sera from Lyme borreliosis patients according to the genomic species of theBorrelia strains used as antigens

  • M. V. Assous
  • D. Postic
  • G. Paul
  • P. Névot
  • G. Baranton
Article

Abstract

Sera of 52 Lyme borreliosis patients classified according to their clinical features were analysed by Western blot using as antigensBorrelia strains belonging to three recently described genomic species. The antibody response was demonstrated to be homologous within each genospecies. Serum reactivity was studied for each of the type strainsBorrelia burgdorferi sensu stricto (strain B31T),Borrelia garinii (strain 20047T) and group VS461. Seven of 15 sera (46.6 %) of patients with menin-goradiculitis showed preferential reactivity withBorrelia garinii (strain 20047T), all of 8 sera (100 %) of patients with acrodermatitis chronica atrophicans showed preferential reactivity with group VS461 (strain VS461) and 8 of 16 sera (50 %) of patients with arthritis showed preferential reactivity withBorrelia burgdorferi sensu stricto (strain B31T). The presence of a strong response to OspA and OspB proteins ofBorrelia burgdorferi sensu stricto was found only in this last group of patients. These results suggest that there are clinical implications of the recently described modifications in the taxonomy ofBorrelia burgdorferi.

Keywords

Arthritis Western Blot Blot Analysis Western Blot Analysis Antibody Response 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1993

Authors and Affiliations

  • M. V. Assous
    • 1
  • D. Postic
    • 2
  • G. Paul
    • 1
  • P. Névot
    • 1
  • G. Baranton
    • 2
  1. 1.Laboratoire de BactériologieFaculté de Médecine CochinParisFrance
  2. 2.Unité de Bactériologie Moléculaire et MédicaleInstitut PasteurParis Cedex 15France

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