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, Volume 15, Issue 5–6, pp 588–593

Cellular distribution of dexamethasone in rabbit spleen and lymph nodes

  • Andrei N. Tchernitchin
  • M. Antonieta Castrillón
  • Angel Rodríguez
Immunosuppression and Inflammation Editorial


The binding of tritiated dexamethasone by rabbit spleen and lymph nodes was studiedin vivo by dry radioautography. The labeled glucocorticoid was found in the surface and cytoplasm of eosinophil leukocytes and in the nuclei of reticular cells, plasmocytes, endothelial cells and macrophages, but not in lymphocytes, lymphoblasts or plasmoblasts. Additional administration of excess of excess of non-radioactive dexamethasone suppressed this labeling. The findings are discussed in relation to the mechanism of action of glucocorticoids in lymphoid organs.


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  1. [1]
    J.D. Boyett andJ.F. Hofert,Studies concerning the inhibition of glucose metabolism in thymus lymphocytes by cortisol and epinephrine, Endocrinology91, 233–239 (1972).PubMedGoogle Scholar
  2. [2]
    D.A. Young,Glucocorticoid action on cat thymus cells. II. Interrelationships between ribonucleic acid and protein metabolism and between cortisol and substrate effects on these metabolic parameters in vitro, J. biol. Chem.245, 2747–2752 (1970).PubMedGoogle Scholar
  3. [3]
    T.F. Dougherty,Effect of hormones on lymphatic tissue, Physiol. Rev.32, 379–401 (1952).PubMedGoogle Scholar
  4. [4]
    W. Niederer,Immunoendokrinologie, Schwiez. med. Wschr.104, 841–847 (1974).Google Scholar
  5. [5]
    N. Sabag, M.A. Castrillón andA. Tchernitchin,Cortisol-induced migration of eosinophil leukocytes to lymphoid organs, Experientia34, 666–667 (1978).CrossRefPubMedGoogle Scholar
  6. [6]
    A. Tchernitchin,The role of eosinophil receptors in the non-genomic response to oestrogens in the uterus, J. Steroid Biochem.11, 417–424 (1979).CrossRefPubMedGoogle Scholar
  7. [7]
    E.V. Jensen andE.R. De Sombre,Mechanism of action of the female sex hormones, A. Rev. Biochem.41, 203–230 (1972).CrossRefGoogle Scholar
  8. [8]
    J.W. Funder andJ.W. Barlow,Heterogeneity of glucocorticoid receptors, Circulation Res.46 Suppl. I, I-83–I-87 (1980).Google Scholar
  9. [9]
    J.D. Baxter, G.G. Rosseau, M.C. Benson, R.L. Garcea, J. Ito andG.M. Tomkins,Role of DNA and specific cytoplasmic receptors in glucocorticoid action, Proc. natn. Acad. Sci. USA69, 1892–1896 (1972).Google Scholar
  10. [10]
    E.B. Thompson andM.E. Lippman,Mechanism of action of glucocorticoids, Metabolism23, 159–202 (1974).CrossRefPubMedGoogle Scholar
  11. [11]
    A. Tchernitchin, X. Tchernitchin, A. Rodriguez, M.A. Mena, C. Unda, N. Mairesse andP. Galand,Effect of propranolol on various parameters of estrogen stimulation in the rat uterus, Experientia33, 1536–1537 (1977).PubMedGoogle Scholar
  12. [12]
    L. Flandroy andP. Galand,Oestrogen-induced increase in uterine cGMP content: a true hormonal action?, Mol. Cell Endocrinol.13, 281–290 (1979).CrossRefPubMedGoogle Scholar
  13. [13]
    B.D. Schaumburg andE. Bojesen,Specificity and thermodynamic properties of the corticosteroid binding to a receptor of rat thymocytes in vitro, Biochim. biophys. Acta171, 172–188 (1968).Google Scholar
  14. [14]
    C. Wira andA. Munck,Specific glucocorticoid receptors in thymus cells. Localization in the nucleus and extraction of the cortisol-receptor complex, J. biol. Chem.245, 3436–3458 (1970).PubMedGoogle Scholar
  15. [15]
    A. Munck, C. Wira, D.A. Young, K.M. Mosher, C. Hallahan andP.A. Bell,Glucocorticoid receptor complexes and the earliest steps in the action of glucocorticoids on thymus cells, J. Steroid Biochem.3, 567–578 (1972).CrossRefPubMedGoogle Scholar
  16. [16]
    W.E. Stumpf andL.J. Roth,High resolution autoradiography with dry mounted, freeze-dried frozen sections. Comparative study of six methods using two diffusible compounds:3 H-estradiol and 3 H-mesobilirubinogen, J. Histochem. Cytochem.14: 274–287 (1966).Google Scholar
  17. [17]
    A. Tchernitchin, L. Tseng, W.E. Stumpf andE. Gurpide,Radioautographic study of human endometrium superfused with estradiol-17β, estrone, estriol and progesterone, J. Steroid Biochem.4, 451–455 (1973).CrossRefPubMedGoogle Scholar
  18. [18]
    A.N. Tchernitchin andN. Tchernitchin,Glucocorticoid localization by radioautography in the rabbit eye following systemic administration of 3 H-dexamethasone, Experientia37, 1120–1121 (1981).PubMedGoogle Scholar
  19. [19]
    Y. Hirasawa andH. Tokuhiro,Electron microscopic studies on the normal human spleen: especially on the red pulp and the reticulo-endothelial cells, Blood35, 201–212 (1970).PubMedGoogle Scholar
  20. [20]
    J.S. Burke andG.T. Simon,Electron microscopy of the spleen. I. Anatomy and microcirculation, Am. J. Path.58, 127–155 (1970).PubMedGoogle Scholar
  21. [21]
    H.K. Müller-Hermelink, V. Heusermann andH.J. Stutte,Enzyme histochemical observation on the localization and structure of the T cell and B cell regions in the human spleen, Cell Tissue Res.154, 167–179 (1974).CrossRefPubMedGoogle Scholar
  22. [22]
    V. Heusermann, H.J. Stutte andH.K. Müller-Hermelink,Interdigitating cells in the white pulp of the human spleen, Cell Tissue Res.153: 415–417 (1974).CrossRefPubMedGoogle Scholar
  23. [23]
    V. Heusermann, K.H. Zurborn, L. Schroeder andH.J. Stutte,The origin of the dendritic reticulum cell. An experimental enzyme-histochemical and electron microscopic study on the rabbit spleen, Cell Tissue Res.209, 279–294 (1980).CrossRefPubMedGoogle Scholar
  24. [24]
    V. Heusermann, L. Schroeder, K.H. Zurborn andH.J. Stutte, Structure and function of stroma cells in lymphoid tissues. InLymphoproliferative Diseases of the Skin, pp. 25–33 (EdsM. Goos andE. Christophers). Springer-Verlag Berlin 1982.Google Scholar
  25. [25]
    A. Tchernitchin andX. Tchernitchin,Characterization of the estrogen receptors in the uterine and blood eosinophil leukocytes, Experientia32, 1240–1242 (1976).CrossRefPubMedGoogle Scholar
  26. [26]
    A. Tchernitchin,Autoradiographic study of (6,7-3 H) oestradiol-17β incorporation into rat uterus, Steroids10, 661–668 (1967).CrossRefPubMedGoogle Scholar
  27. [27]
    R.J. Pietras andC.M. Szego,Partial purification and characterization of oestrogen receptors in subfractions of hepatocyte plasma membranes, Biochem. J.191, 743–760 (1980).PubMedGoogle Scholar
  28. [28]
    A.P. Peterson, L.C. Altman, J.S. Hill, K. Gosney andM.E. Kadin,Glucocorticoid receptors in normal human eosinophils: comparison with neutrophils, J. Allergy Clin. Immunol.68, 212–217 (1981).PubMedGoogle Scholar
  29. [29]
    X. Tchernitchin, A. Tchernitchin andP. Galand,Dynamics of eosinophils in the uterus after oestrogen administration, Differentiation5, 151–154 (1976).CrossRefPubMedGoogle Scholar
  30. [30]
    M.A. Castrillón, N. Sabag andA.N. Tchernitchin,Effect of splenectomy in cortisol-induced lymphoid tissue eosinophilia, IRCS Med. Sci.8, 467 (1980).Google Scholar

Copyright information

© Birkhäuser Verlag 1984

Authors and Affiliations

  • Andrei N. Tchernitchin
    • 1
  • M. Antonieta Castrillón
    • 1
  • Angel Rodríguez
    • 1
  1. 1.Laboratory of Experimental Endocrinology, Department of Experimental MorphologyUniversity of Chile Medical SchoolSantiagoChile

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