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, Volume 14, Issue 2, pp 300–305 | Cite as

Platelet activating factor: Regulation by mast cells and aspirin

  • J. A. Denburg
  • D. B. Williams
  • R. L. Kinlough-Rathbone
  • J. -P. Cazenave
  • J. Bienenstock
Platelets and Thrombosis

Abstract

We have investigated some aspects of the regulation of production of rat platelet activating factor (PAF)in vitro. Suspensions of unseparated (PLC1), mast cell-depleted (PLC2), or mast cell (MC)-enriched rat peritoneal lavage cells (PLC) were analyzed for PAF content by extraction at alkaline pH. PAF activity extracted from PLC1 varied inversely with viable cell concentration: at 1×106 cells/ml, 32±9.3 PAF units, decreasing to 11.2±9.5 units at 10×106 cells/ml, and no activity at higher concentrations. Incubation of PLC1 in Tyrode's buffer or acetylsalicylic acid (ASA), but not salicylate, resulted in a time-dependent loss of PAf activity. Mean PAF activity of PLC2 was similar to that in PLC1, while no PAF activity was extractable from MC. Co-incubation with MC extracts inhibited PAF activity of PLC1 extracts in a dose-dependent fashion. Ultracentrifugation of PAF-containing samples led to a loss of all PAF activity in PLC1 extracts, suggesting the association of PAF activity with subcellular components. PAF appears to be derived from a non-MC population of rat PLC, is not extractable from rat PLC in the presence of ASA and is inhibited by MC extracts. These studies suggest that ASA regulates PAF availability unrelated to its effect on cylcooxygenase and that MC membrane products directly inhibit PAF activity from rat PLC.

Keywords

Aspirin Mast Cell Salicylate Cell Concentration Acetylsalicylic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Birkhäuser-Verlag 1984

Authors and Affiliations

  • J. A. Denburg
    • 1
  • D. B. Williams
    • 2
  • R. L. Kinlough-Rathbone
    • 2
  • J. -P. Cazenave
    • 3
  • J. Bienenstock
    • 2
  1. 1.Department of MedicineMcMaster University Health Sciences CentreHamiltonCanada
  2. 2.Department of PathologyMcMaster UniversityHamiltonCanada
  3. 3.Centre de Transfusion SanguineStrasbourg CedexFrance

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