Agents and Actions

, Volume 12, Issue 1–2, pp 60–63

Histamine release from human pulmonary mast cells

  • Madeleine Ennis
Histamine and Kinins Histamine Release

Abstract

The enzyme collagenase was used to disperse human lung into its component cells. The resulting cell suspensions containedcirca 8% mast cells and were used for studies of mediator release without further purification. They exhibited a low (circa 7%) spontaneous release of histamine. They could be sensitized passively and released histamine upon challenge with anti-human IgE. They responded to concanavalin A but not to dextran. Phosphatidyl serine did not potentiate the release induced by these agents. The calcium ionophores, A23187 and ionomycin, both elicited histamine release. The cells were refractory to the action of the basic releasers 48/80 and peptide 401 (MCD-peptide). These results indicate marked differences between human pulmonary mast cells and the more widely used rat peritoneal mast cells.

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References

  1. [1]
    F.L. Pearce andM. Ennis,Isolation and some properties of mast cells from the mesentery of the rat and guinea-pig, Agents and Actions10, 124–131 (1980).PubMedGoogle Scholar
  2. [2]
    M. Ennis andF.L. Pearce,Differential reactivity of isolated mast cells from the rat and guinea-pig, Eur. J. Pharmac.66, 339–345 (1980).CrossRefGoogle Scholar
  3. [3]
    W. Schmutzler, F. Thiele, U. Rosenkranz, U. Blum, F.L. Pearce, G. Poblete-Freund andP. Pult,Studies on isolated mast cells from guinea-pig, dog, calf and man, Agents and Actions8, 407 (1978).PubMedGoogle Scholar
  4. [4]
    M. Ennis, A. Truneh andF.L. Pearce,Lectin-induced histamine secretion from isolated rat and guinea-pig mast cells, Biochem. Pharmacol.30, 2179–2181 (1981).CrossRefPubMedGoogle Scholar
  5. [5]
    N.A.M. Patterson, S.I. Wasserman, J.W. Said andK.F. Austen,Release of chemical mediators from partially purified human lung mast cells, J. Immunol.117, 1356–1361 (1976).PubMedGoogle Scholar
  6. [6]
    M. Ennis, A. Truneh, J.R. White andF.L. Pearce,Calcium pools involved in histamine release from mast cells, Int. Archs Allergy Appl. Immun.62, 467–471 (1980).Google Scholar
  7. [7]
    F.L. Pearce,The use of porous plastic filters in the preparation of cell suspensions, Microscopica Acta79, 43–45 (1977).PubMedGoogle Scholar
  8. [8]
    I. Mota andI. Vugman,Effects of anaphylactic shock and compound 48/80 on the mast cells of the guinea-pig lung, Nature177, 427–429 (1956).PubMedGoogle Scholar
  9. [9]
    P.A. Shore, A. Burkhalter andV.H. Cohn,A method for the fluorometric assay of histamine in tissues, J. Pharmac. Exp. Ther.127, 182–186 (1959).Google Scholar
  10. [10]
    J.L. Mongar andH.O. Schild,Inhibition of the anaphylactic reaction, J. Physiol.135, 301–319 (1957).PubMedGoogle Scholar
  11. [11]
    J.R. Carstairs, M.M. Dale andJ.L. Mongar,The isolation and some properties of guinea-pig mesenteric mast cells, Exp. Cell Res.81, 156–162 (1973).CrossRefPubMedGoogle Scholar
  12. [12]
    K.E. Barrett andF.L. Pearce,A comparative study of histamine secretion from rat peritoneal and pleural mast cells, Agents and Actions (in press).Google Scholar
  13. [13]
    K.W. Batchelor andD.R. Stanworth,A comparison of the histamine-releasing properties of rat pleural and peritoneal mast cells, Immunology41, 271–278 (1980).PubMedGoogle Scholar
  14. [14]
    R.P. Siraganian andK.A. Hazard,Mechanisms of mouse mast cell activation and inactivation for IgE-mediated histamine release, J. Immunol.122, 1719–1725 (1979).PubMedGoogle Scholar
  15. [15]
    J.P. Bennett, S. Cockcroft andB.D. Gomperts,Ionomycin stimulates mast cell histamine secretion by forming a lipid-soluble calcium complex, Nature282, 851–853 (1979).PubMedGoogle Scholar
  16. [16]
    A.H. Soll, K. Lewin andM.A. Beaven,Isolation of histamine-containing cells from canine fundic mucosa, Gastroenterology77, 1283–1290 (1979).PubMedGoogle Scholar
  17. [17]
    A.D. Befus, F.L. Pearce, J. Gauldie, P. Horsewood, R.L. Goodacre, F. Cole, R.V. Heatley andJ. Bienenstock, Isolation and characteristics of mast cells from the lamina propria of the small bowel. InThe Mast Cell, its Role in Health and Disease, pp. 702–709 (EdsJ. Pepys andA.M. Edwards). Pitman Medical, Tunbridge Wells 1979.Google Scholar
  18. [18]
    F.L. Pearce, A.D. Befus andJ. Bienenstock,Isolation and properties of mast cells from the small bowel lamina propria of the rat, Agents and Actions (in press).Google Scholar

Copyright information

© Birkhäuser Verlag 1982

Authors and Affiliations

  • Madeleine Ennis
    • 1
  1. 1.The Department of Clinical PharmacologyRoyal Postgraduate Medical School, Hammersmith HospitalLondonUK

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