Agents and Actions

, Volume 12, Issue 1–2, pp 26–29

Simultaneous fluorometric assay of histamine and histidine in biological fluid using an automated analyser

  • E. S. K. Assem
  • E. K. S. Chong
Histamine and Kinins Determination of Histamine and Its Metabolites

DOI: 10.1007/BF01965101

Cite this article as:
Assem, E.S.K. & Chong, E.K.S. Agents and Actions (1982) 12: 26. doi:10.1007/BF01965101


A sensitive fluorometric method for the assay of histamine and histidine was developed. The method allows for the automated simultaneous assay of the release of these substances from tissue and blood cell preparations in immune and non-immune reactions. The sensitivity for histamine and histidine were 0.5 ng and 10 ng per ml respectively. The assay procedure for histidine, however, was not as specific as that for histamine.

Copyright information

© Birkhäuser Verlag 1982

Authors and Affiliations

  • E. S. K. Assem
    • 1
  • E. K. S. Chong
    • 1
  1. 1.Department of PharmacologyUniversity College LondonLondonUK

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