Histamine and acute haemorrhagic lesions in rat gastric mucosa: Prevention of stress ulcer formation by (+)-catechin, an inhibitor of specific histidine decarboxylase in vitro
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Acute haemorrhagic lesions in the oesophagus, stomach and duodenum (‘stress ulcers’) occur relatively often under clinical conditions and are always dangerous to the patient (lethality rate about 70%). Since conservative and surgical treatment are without significant success up to now, prevention by adaptation to stressors or by administration of drugs seems mandatory.
An improved technique for producing acute gastric lesions in rats by immobilization and a new method for assessing this disease in the animals is presented in this communication. High precision is obtained within a single experimental series especially from day to day.
Since histamine was suggested to be involved in the pathogenesis of stress ulcer disease, (+)-catechin, a rather specific inhibitor of specific histidine decarboxylase from rat stomach, was tested in immobilized rats. It prevented the formation of acute gastric lesions by 80% in seven series of experiments lasting for half a year. Since the drug has low toxicity in man, it is recommended for clinical trials.
KeywordsHistamine Histidine Clinical Condition High Precision Gastric Mucosa
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- K.-Fr. Sewing andB. Binder,The Effect of Brocresine (NSD-1055) on Diamine Oxidase Levels in the Rat Plasma, Liver, Stomach and Upper Small Intestine in vivo and vitro, in:Histamine (Ed. Cz. Maslinski; Dowden, Hutchinson & Ross, Stroudsburg, Pennsylvania, 1973), p. 306.Google Scholar
- W. Lorenz, J. Kusche, H. Barth andCh. Mathias,Action of Several Flavonoids on Enzymes of Histamine Metabolism in vitro, in:Histamine (Ed. Cz. Maslinski; Dowden, Hutchinson & Ross, Stroudsburg, Pennsylvania, 1973), p. 265.Google Scholar
- W. Lorenz, H.-J. Reimann, J. Kusche, H. Barth, A. Schmal, H. Nusimé, G. Schülle, R. Froelich, J. Schmidt andR. Raabe,Effects of (+)-Catechin on Several Enzymes of Histamine Metabolism and on Stress Ulcer Formation in the Female Rat, Naunyn-Schmiedeberg's Arch. Pharmacol. Suppl.287, 62 (1975).Google Scholar
- G.W. Snedecor andW.G. Cochran,Statistical Methods (Iowa State University Press, 1967), p. 62.Google Scholar
- H.U. Bergmeyer,Methods of Enzymatic Analysis, sec. Engl. Ed., Chemie Weinheim (Academic Press, Inc., New York and London, 1974).Google Scholar
- S. Yano andM. Haraoa,A Method for the Production of Stress Erosion in the Mouse Stomach and Related Pharmacological Studies, Japan. J. Pharmacol.23, 57 (1973).Google Scholar
- S. Sethbhakdi, C.J. Pfeiffer andJ.L.A. Roth,Gastric Mucosal Ulceration Following Vasoactive Agents. A New Experimental Model, Digestive Diseases15, 261 (1970).Google Scholar
- A. Robert, J.I. Northam, I.E. Nezamir andI.D. Phillips,Exertion Ulcers in the Rat, Digestive Diseases15, 497 (1970).Google Scholar
- W. Lorenz, H. Barth, J. Kusche, H. Troidl, H. Rohde, A. Schmal, W. Stenner, H. Nusime, G. Häfner, R. Glass andA. Friedrich,Critical Review Concerning Metabolism and Physiological Function of Histamine in the Gastric Mucosa, Satellite Symposium on Gastrointestinal Hormones, Krakow 1974 (in press).Google Scholar
- M.N. Gleason, R.E. Gosselin andH.C. Hodge,Clinical Toxicology of Commercial Products (Williams and Wilkins Company, Baltimore, 1963).Google Scholar