Comparative evaluation of a latex test for the identification ofStaphylococcus aureus

  • M. Stevens
  • C. Geary


A rapid latex agglutination test, Staphaurex, was tested for its ability to identifyStaphylococcus aureus using 72 reference strains and 785 clinical isolates of the familyMicrococcaceae. All reference strains ofStaphylococcus aureus were Staphaurex-positive. Non-Staphylococcus aureus reference strains were negative. Using clinical strains, the results of the Staphaurex test were compared with the results of other tests commonly used to identifyStaphylococcus aureus. A total of 393 clinical isolates were classified asStaphylococcus aureus. The Staphaurex, slide coagulase, tube coagulase/human plasma and tube coagulase/rabbit plasma tests correctly identified 98%, 93.6%, 93.6% and 97.5% of theStaphylococcus aureus strains, respectively. The performance of the Staphaurex test, in terms of sensitivity and specificity, was significantly better than the slide coagulase test. It was as sensitive and almost as specific as the tube coagulase rabbit test and more sensitive than the tube coagulase human test.


Internal Medicine Clinical Isolate Reference Strain Comparative Evaluation Clinical Strain 
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  1. 1.
    Orth, D. S., Chugg, L. R., Anderson, A. W.: Comparison of animal sera for suitability in coagulase testing. Applied Microbiology 1971, 21: 420–425.Google Scholar
  2. 2.
    Menzies, R. E.: Comparison of coagulase, deoxyribonuclease (DNase) and heat stable nuclease tests for the identification ofStaphylococcus aureus. Journal of Clinical Pathology 1977, 30: 606–608.Google Scholar
  3. 3.
    Lachica, R. V. F., Genigeorgis, C., Hoeprich, P. D.: Metachromatic agar-diffusion methods for detecting staphylococcal nuclease activity. Applied Microbiology 1971, 21: 585–587.Google Scholar
  4. 4.
    Barrett, S. P.: Rate of isolation ofStaphylococcus aureus strains possessing coagulase or clumping factor. European Journal of Clinical Microbiology 1983, 2: 475–476.Google Scholar
  5. 5.
    Essers, L., Radebold, K.: Rapid and reliable identification ofStaphylococcus aureus by a latex agglutination test. Journal of Clinical Microbiology 1980, 12: 641–643.Google Scholar
  6. 6.
    Maxim, P. E., Mathews, H. L., Mengoli, H. F.: Singletube mixed agglutination test for the detection of staphylococcal protein A. Journal of Clinical Microbiology 1976, 4: 418–422.Google Scholar
  7. 7.
    Feltham, R. K. A.: A taxonomic study of theMicrococcaceae. Journal of Applied Bacteriology 1979, 47: 243–254.Google Scholar
  8. 8.
    Geary, C., Stevens, M.: Rapid lysostaphin test to differentiateStaphylococcus andMicrococcus species. Journal of Clinical Microbiology 1986, 23: 1044–1045.Google Scholar
  9. 9.
    Pennell, D. R., Rott-Petri, J. A., Kurzynski, T. A.: Evaluation of three commercial agglutination tests for the identification ofStaphylococcus aureus. Journal of Clinical Microbiology 1984, 20: 614–617.Google Scholar
  10. 10.
    Landau, W., Kaplan, R. L.: Room temperature coagulase production byStaphylococcus aureus strains. Clinical Microbiology Newsletter 1980, 2: 10.Google Scholar
  11. 11.
    Cadness-Graves, B., Williams, R., Harper, G. H., Miles, A. A.: A slide test for coagulase-positive staphylococci. Lancet 1943, i: 736–738.Google Scholar
  12. 12.
    Dickson, J. I. S., Marples, P. R.: Coagulase production by strains ofStaphylococcus aureus of differing resistance characters: a comparison of two traditional methods with a latex agglutination system detecting both clumping factor and protein A. Journal of Clinical Pathology 1986, 39: 371–375.Google Scholar
  13. 13.
    Berke, A., Tilton, R. C.: Evaluation of rapid coagulase methods for the identification ofStaphylococcus aureus. Journal of Clinical Microbiology 1986, 23: 916–919.Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1989

Authors and Affiliations

  • M. Stevens
    • 1
  • C. Geary
    • 1
  1. 1.Public Health LaboratoryRoyal InfirmaryLeicesterUK

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