Experientia

, Volume 35, Issue 9, pp 1261–1262 | Cite as

Measurement of urinary steroid production rates using stable-isotopes and GC-MS

  • D. W. Johnson
  • G. Phillipou
  • I. A. Blair
  • R. F. Seamark
Specialia

Summary

The urinary production rate of pregnenolone has been determined for a male subject using 7,7-d2-pregnenolone as an isotopic tracer.

Keywords

Steroid Production Rate Male Subject Pregnenolone Isotopic Tracer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in to check access.

References

  1. 2.
    I.A. Blair, G. Phillipou and C.J. Seaborn, J. label. Comp. Radiopharm.15, 645 (1978).Google Scholar
  2. 3.
    G. Phillipou, R.F. Seamark and L.W. Cox, Aust. N.Z. J. Med.8, 63 (1978).Google Scholar
  3. 4.
    G. Phillipou, R.F. Seamark and R. Sweet, Aust. N.Z. J. Obstet. Gynec., in press.Google Scholar
  4. 5.
    F. Berthou, L. Bardou and H.H. Floch, J. Chromat.93, 149 (1974).Google Scholar
  5. 6.
    Pentafluoroacyl derivatives were chosen to minimise the natural isotopic contribution at (M+2) from the endogenous steroid.Google Scholar
  6. 7.
    F.C. Falkner, B.J. Sweetman and J.T. Watson, Appl. Spectrosc. Rev.10, 5 (1975).Google Scholar
  7. 8.
    L.P. Romanoff, M.P. Grace, M.N. Baxter and G. Pincus, J. clin. Endocr. Metab.26, 1023 (1966).Google Scholar
  8. 9.
    L.P. Romanoff and H.J. Brodie, J. Steroid Biochem.7, 289 (1976).Google Scholar
  9. 10.
    For a complete discussion on this type of dynamic steroid interconversion and its implications on production rate calculations see reference 12.Google Scholar
  10. 11.
    J.L. Pinkus, D. Charles and S.C. Chattoraj, J. biol. Chem.246, 633 (1971).Google Scholar
  11. 12.
    R.L. Vande Wiele, P.C. MacDonald, E. Gurpide and S. Lieberman, Rec. Prog. Hormone Res.19, 275 (1963).Google Scholar

Copyright information

© Birkhäuser Verlag 1979

Authors and Affiliations

  • D. W. Johnson
    • 1
  • G. Phillipou
    • 1
  • I. A. Blair
    • 1
  • R. F. Seamark
    • 1
  1. 1.Endocrine Laboratory, Department of Obstetrics and GynaecologyThe Queen Elizabeth HospitalWoodville

Personalised recommendations