Pharmaceutisch Weekblad

, Volume 6, Issue 3, pp 105–110 | Cite as

Comparison of media and temperature of incubation for microbiological examination of purified water

  • H. Van Doorne
  • A. Neuteboom
Original Articles


The suitability of four commonly used nutrient media, and two incubation temperatures for the bacteriological examination of purified water was investigated. The temperature of incubation (22 or 30°C) exerted no profound influence on the observed numbers of colony forming units. Five days was optimal for all incubation conditions. Tryptone Soya Peptone Broth Agar was found to be less productive than the other media investigated,viz. Nutrient Agar 1/8 strength. Nutrient Agar and King A medium, which appeared to be equally productive.


Public Health Internal Medicine Agar Peptone Nutrient Medium 
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  1. 1.
    United States Pharmacopoeia 20th ed. Rockville: United States Pharmacopoeial Convention Inc., 1980:851.Google Scholar
  2. 2.
    Nederlandse Farmacopee 8th ed. 's-Gravenhage: Staatsuitgeverij, 1978:153.Google Scholar
  3. 3.
    King EO, Ward MK, Raney DE. Two simple media for the demonstration of pyocyanin and fluorescence. J Lab Clin Med 1954;44:301–7.Google Scholar
  4. 4.
    Van der Kooy D. Vermeerdering van bacteriën in drinkwater. H2O 1981;14:317–23.Google Scholar
  5. 5.
    Abdou MA-F. In Pharmazeutika unerwünschte Mikro-Organismen I.Pseudomonas aeruginosa: Bedeutung, Vorkommen, Nachweis, und Bekämpfung. Pharm Ind 1973;35:857–67.Google Scholar
  6. 6.
    Mossel DAA, Ratto MA. Wholesomeness of some types of semi-preserved foods. J Food Technol 1973;8:97–103.Google Scholar
  7. 7.
    Wilcoxon F. Individual comparisons by ranking methods. Biometrics Bull 1945;51:80–3.Google Scholar
  8. 8.
    Morita RY. Psychrophilic Bacteria. Bact Rev 1975;39:144–67.Google Scholar
  9. 9.
    Ingram M, Mackey BM. Inactivation by cold. In: Skinner FA, Hugo WB, eds. Inhibition and inactivation of vegetative microbes. London: Academic Press, 1976;111–51.Google Scholar
  10. 10.
    Bruining M, Beekhuizen CCM, Hoevenaars PCM. Onderzoek naar de microbiologische kwaliteit van gedestilleerd water uit verschillende apotheken. Pharm Weekbl 1972;107:321–8.Google Scholar
  11. 11.
    Eisman PC, Kull FC, Mayer RL. The bacteriological aspects of deionized water. J Am Pharm Ass [Sci] 1949;38:88–91.Google Scholar
  12. 12.
    Faber DB, Meester HH. Microbiologische zuiverheid van demiwater. Pharm Weekbl 1972;107:329–36.Google Scholar
  13. 13.
    Leifson E. The bacterial flora of distilled and stored water. I. General observations, techniques and ecology. Int Bull Bact Nomencl Taxonomy 1962;12:133–53.Google Scholar
  14. 14.
    Leifson E. The bacterial flora of distilled and stored water. II.Caulobacter vibrioides, Henrici and Johnson 1935, in distilled water. Intern Bull Bact Nomencl Taxonomy 1962;12:155–9.Google Scholar
  15. 15.
    Leifson E. The bacterial flora of distilled and stored water. III. New species of the generaCorynebacterium, Flavobacterium, Spirillum, Pseudomonas. Int Bull Bact Nomencl Taxonomy 1962;12:161–70.Google Scholar
  16. 16.
    Wachi Y, Yanagi M, Katsura H, Ohta S. Decomposition of surface active agents by bacteria isolated from deionized water. J Soc Cosm Chem 1980;31:67–84.Google Scholar
  17. 17.
    Zimmermann G, Krüger D, Busse M. Die Bakterienflora von destilliertem und vollentsalztem Wasser und ihre Bedeutung bei der Herstellung von Arzneimitteln. Pharm Ind 1980;42:932–9.Google Scholar
  18. 18.
    Schure PSJ. Microbiologische zuiverheid van gedestilleerd water uit apotheken. Ned Tijdschr Geneeskd 1970;114:591–3.Google Scholar
  19. 19.
    Schure PSJ. Het bereiden en bewaren van gedestilleerd water voor de receptuur. Pharm Weekbl 1974;109:597–603.Google Scholar
  20. 20.
    Bühlmann X. Psychrophile Keime im Wasser: Verhalten und praktische Bedeutung. Alimenta 1970;9:22–9.Google Scholar
  21. 21.
    Postgate JR, Hunter JR. Accelerated death ofAerobacter aerogenes starved in the presence of growthlimiting substrates. J Gen Microbiol 1964;34:459–73.Google Scholar
  22. 22.
    Abdou MA-F. Sterilitätstest. Pharm Ind 1973;35:650–8.Google Scholar
  23. 23.
    Clausen OG. A study of the growth promoting properties of fluid and solid microbial-contamination test media on small numbers of micro-organisms. Pharm Acta Helv 1973;48:541–8.Google Scholar
  24. 24.
    Clausen OG, Aasgaard HB, Solberg O. Dithionitethioglycollate broth (HS-T Broth) a new control medium for microbial contamination tests of medical products. Ann Microbiol (Paris) 1973;124B:205–16.Google Scholar
  25. 25.
    Hein H, Neiss A. Vergleich verschiedener Nährlösungen zur Züchtung von Hefen. Pharm Ind 1971;33:75–8.Google Scholar
  26. 26.
    Bowman FW. The sterility testing of pharmaceuticals. J Pharm Sci 1969;58:1301–8.Google Scholar
  27. 27.
    Van Elburg-van Delft R, Mossel DAA, Corstiaensen GP, Van Ederveen W. An investigation into the nature and determination of the ‘total’ bacterial colonization of water of drinking water quality. Water Res, in press.Google Scholar

Copyright information

© Royal Dutch Association for Advancement of Pharmacy 1984

Authors and Affiliations

  • H. Van Doorne
    • 1
  • A. Neuteboom
    • 1
  1. 1.Department of Pharmaceutical Technology, Subfaculty of PharmacyState University of LeidenRA LeidenThe Netherlands

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