In vitro activity of sulphamethoxazole/trimethoprim and sulfadoxine/pyrimethamine againstChlamydia trachomatis SA2f in McCoy cell culture

  • G. Mumtaz
  • G. L. Ridgway
  • D. Felmingham


Chequerboard titrations of sulphamethoxazole/trimethoprim and sulfadoxine/pyrimethamine were performed againstChlamydia trachomatis (strain SA2f) using McCoy cell monolayers in vials. The experiments were continued for ten passages each. The mean fractional inhibitory concentration index for each combination was calculated. Results demonstrated synergistic activity between sulphamethoxazole and trimethoprim, and between sulfadoxine and pyrimethamine.


Cell Culture Internal Medicine Inhibitory Concentration Cell Monolayer Trimethoprim 
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  1. 1.
    How, S. J., Hobson, D., Hart, C. A., Webster, R. E. An in vitro investigation of synergy and antagonism between antimicrobials againstChlamydia trachomatis. Journal of Antimicrobial Chemotherapy 1985, 15: 533–538.PubMedGoogle Scholar
  2. 2.
    Ridgway, G. L., Owen, J. M., Oriel, J. D. A method for testing the antimicrobial sensitivity ofChlamydia trachomatis in cell culture system. Journal of Antimicrobial Chemotherapy 1976, 2: 77–81.PubMedGoogle Scholar
  3. 3.
    Ridgway, G. L., Owen, J. M., Oriel, J. D. The antimicrobial susceptibility ofChlamydia trachomatis in cell cultures. British Journal of Venereal Diseases 1978, 54: 103–106.PubMedGoogle Scholar
  4. 4.
    Krogstad, D. J., Moellering, R. C. Antimicrobial combinations. In: Lorion, V. (ed.): Antibiotics in laboratory medicine. Williams and Wilkins, Baltimore, 1986, p. 537–595.Google Scholar
  5. 5.
    Hammerschlag, M. R. The activity of trimethoprim-sulphamethoxazole againstChlamydia trachomatis in vitro. Review of Infectious Diseases 1982, 4: 500–505.Google Scholar
  6. 6.
    Johannison, G., Sernryd, A., Lyceke, L. Susceptibility ofChlamydia trachomatis to antibiotics in vitro and in vivo. Sexually Transmitted Diseases 1979, 6: 50–57.PubMedGoogle Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1988

Authors and Affiliations

  • G. Mumtaz
    • 1
  • G. L. Ridgway
    • 1
  • D. Felmingham
    • 1
  1. 1.Department of Clinical MicrobiologyUniversity College HospitalLondonUK

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