Pharmaceutisch Weekblad

, Volume 5, Issue 3, pp 95–101 | Cite as

HPLC determination of chloramphenicol degradation in eye drops

  • Y. Boer
  • A. Pijnenburg
Original Articles


Anhplc-method is described for the simultaneous determination of chloramphenicol and its most important degradation products. A 15 cm long C-18 reversed phase column was used with a mobile phase consisting of boric acid solution-acetonitrile (60+45) adjusted to a pH of 3. The amount of degradation of chloramphenicol eye drop solutions containing boric acid and borax, at pH 4.7 and 7.2 respectively, is determined at 4, 21, 100 and 120°C. The solution of pH 7.2 is more stable than that of pH 4.7 at 4 and 21°C, but is less stable at 100 and 120°C. Preparation has to be accomplished in subdued light. The solutions have to be protected from light during storage.


Public Health Internal Medicine Mobile Phase Degradation Product Chloramphenicol 
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Copyright information

© Royal Dutch Association for Advancement of Pharmacy 1983

Authors and Affiliations

  • Y. Boer
    • 1
  • A. Pijnenburg
    • 1
  1. 1.Laboratory of the Dutch PharmacistsJL The HagueThe Netherlands

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