Experientia

, Volume 47, Issue 5, pp 483–485

Mitochondrial DNA variation in geographic populations of Colorado potato beetle,Leptinotarsa decemlineata (Coleoptera; Chrysomelidae)

  • A. M. L. Azeredo-Espin
  • R. F. W. Schroder
  • M. D. Huettel
  • W. S. Sheppard
Research Articles

DOI: 10.1007/BF01959950

Cite this article as:
Azeredo-Espin, A.M.L., Schroder, R.F.W., Huettel, M.D. et al. Experientia (1991) 47: 483. doi:10.1007/BF01959950

Summary

This study demonstrates variability in restriction enzyme cleavage sites of mitochondrial DNA (mtDNA) among four popalations of Colorado potato beetle (CPB). A suite of three enzymes (EcoRI,HpaI,PstI) was sufficient to discriminate among the populations tested. Individuals heteroplasmic for restriction enzyme patterns were found in some populations. Variability in CPB mtDNA should prove useful in efforts to trace the origin and dispersal of the species in North America.

Key words

Mitochondrial DNA RFLP Leptinotarsa decemlineata Colorado potato beetle population genetics 

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Copyright information

© Birkhäuser Verlag 1991

Authors and Affiliations

  • A. M. L. Azeredo-Espin
    • 1
  • R. F. W. Schroder
    • 1
  • M. D. Huettel
    • 1
  • W. S. Sheppard
    • 2
  1. 1.Insect Biocontrol LaboratoryARS-USDABeltsvilleUSA
  2. 2.Bee Research LaboratoryARS-USDABeltsvilleUSA
  3. 3.Depto de Genetica e EvolucaoUniversidade Estadual de Campinas, IB-UNICAMPBrasil

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