Prothymosin alpha is not a nuclear polypeptide
Article
Received:
Accepted:
- 9 Downloads
- 15 Citations
Summary
Using a radioimmunoassay for the NH2-terminus of prothymosin alpha, the crossreactive material was measured in subcellular fractions of calf thymus and liver. No significant amount of crossreactive material was found in the nucleus. This provides experimental evidence against a recent hypothesis, based on structural evidence, that prothymosin alpha is a nuclear polypeptide.
Key words
Prothymosin alpha thymosin alpha 1Preview
Unable to display preview. Download preview PDF.
References
- 1.Haritos, A. A., Blacher, R., Stein, S., Caldarella, J., and Horecker, B. L., Proc. natl Acad. Sci. USA82 (1985) 343.PubMedGoogle Scholar
- 2.Pan, L.-X., Haritos, A. A., Wideman, J., Komiyama, T., Chang, M., Stein, S., Salvin, S. B., and Horecker, B. L., Archs Biochem. Biophys.250 (1986) 197.CrossRefGoogle Scholar
- 3.Goodall, G. J., Dominguez, F., and Horecker, B. L., Proc. natl Acad. Sci. USA83 (1986) 8926.PubMedGoogle Scholar
- 4.Eschenfeldt, W. H., and Berger, S. L., Proc. natl Acad. Sci. USA83 (1986) 9403.PubMedGoogle Scholar
- 5.Panneerselvam, C., Wellner, D., and Horecker, B. L., Archs Biochem. Biophys.265 (1988) 454.CrossRefGoogle Scholar
- 6.Haritos, A. A., Tsolas, O., and Horecker, B. L., Proc. natl Acad. Sci. USA81 (1984) 1391.PubMedGoogle Scholar
- 7.Haritos, A. A., Caldarella, J., and Horecker, B. L., Analyt. Biochem.144 (1985) 436.CrossRefPubMedGoogle Scholar
- 8.Panneerselvam, C., Haritos, A. A., Caldarella, J., and Horecker, B. L., Proc. natl Acad. Sci. USA84 (1987) 4465.PubMedGoogle Scholar
- 9.Haritos, A. A., in: Isozymes: Current Topics in Biological and Medical Research, vol. 14, p. 123. Eds M. C. Ratazzi, J. G. Scandalios and G. Whitt. Alan R. Liss Inc., New York 1987.Google Scholar
- 10.Yialouris, P. P., Evangelatos, G. P., Soteriadis-Vlahos, C., Heimer, E. P., Felix, A. M., Tsitsiloni, O. E., and Haritos, A. A., J. immun. Meth.106 (1988) 267.CrossRefGoogle Scholar
- 11.Haritos, A. A., Salvin, S., Blacher, R., Stein, S., and Horecker, B. L., Proc. natl Acad. Sci. USA82 (1985) 1050.PubMedGoogle Scholar
- 12.Baxevanis, C. N., Reclos, G. J., Papamichail, M., and Tsokos, G. C., Immunopharmac. Immunotoxic.9 (1987) 429.Google Scholar
- 13.Reclos, G. J., Baxevanis, C. N., Sfagos, C., Papageorgiou, C., Tsokos, G. C., and Papamichail, M., Clin. exp. Immun.70 (1987) 336.PubMedGoogle Scholar
- 14.Gómez-Márquez, J., and Segade, F., FEBS Lett.226 (1988) 217.CrossRefPubMedGoogle Scholar
- 15.Higashi, K., Narayanan, K. S., Adams, H. R., and Busch, H., Cancer Res.26 (1966) 1582.PubMedGoogle Scholar
- 16.Haritos, A. A., Goodall, G. J., and Horecker, B. L., Proc. natl Acad. Sci. USA81 (1984) 1008.PubMedGoogle Scholar
- 17.Tsitsiloni, O. E., Yialouris, P. P., Heimer, E. P., Felix, A. M., Evangelatos, G. P., Soteriadis-Vlahos, C., Stiakakis, J., Hannappel, E., and Haritos, A. A., J. immun. Meth.113 (1988) 175.CrossRefGoogle Scholar
- 18.Stahli, C., Takacs, B., and Kocyba, C., Molec. Immun.20 (1983) 20.Google Scholar
- 19.Auger, C., Stahli, C., Fabien, N., and Monier, J. C., J. Histochem. Cytochem.35 (1987) 181.PubMedGoogle Scholar
- 20.Fabien, N., Auger, C., and Monier, J. C., Immunology63 (1988) 721.PubMedGoogle Scholar
- 21.Horecker, B. L., and Morgan, J., in: Lymphokines, vol. 9, p. 15. Eds E. Pick and M. Landy. Academic Press, New York 1984.Google Scholar
- 22.McClure, J. E., Lameris, N., Wara, D. W., and Goldstein, A. L., J. Immun.128 (1981) 368.Google Scholar
- 23.Hannappel, E., and Van Kampen, M., J. Chromat.397 (1987) 279.CrossRefGoogle Scholar
Copyright information
© Birkhäuser Verlag Basel 1989