Experientia

, Volume 38, Issue 12, pp 1441–1442 | Cite as

Physicochemical studies of indocyanine green (ICG): absorbance/concentration relationship, pH tolerance and assay precision in various solvents

  • Ó. G. Björnsson
  • R. Murphy
  • V. S. Chadwick
Short Communications Chemistry, Physics, Biomathematics

Summary

Indocyanine green (ICG) obeyed the Beer-Lambert law within the concentration range 1.25 μg/ml–10.0μg/ml in distilled water, methanol, dimethylformamide (DMF), 1.2-propanediol and aqueous buffers (pH 9.0), but only up to 7.5 μg/ml in human bile and 0.5% human albumin, and only to 5.0 μg/ml in human duodenal fluid. ICG was rapidly (<1 h) decomposed to a colorless derivative at pH<5 and >11, but remained relatively stable for 48 h at pH 8–10. ICG is an indicator and a weak acid with a pKa of 3.27. In bile stabilized with 25% methanol, the precision of the method (CV) is 5% and the accuracy is 106–127%.

Keywords

Methanol Albumin Dimethylformamide Weak Acid Indocyanine Green 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Birkhäuser Verlag 1982

Authors and Affiliations

  • Ó. G. Björnsson
    • 1
  • R. Murphy
    • 1
  • V. S. Chadwick
    • 1
  1. 1.Royal Postgraduate Medical SchoolLondonHammersmith HospitalEngland

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