Pharmaceutisch Weekblad

, Volume 6, Issue 2, pp 75–79 | Cite as

A new and improved gas Chromatographic method for the determination of fluoride in plasma and faeces

  • J. H. Glerum
  • A. van Dijk
  • S. W. Klein
Original Articles


A method is described that extracts fluoride selectively as trimethylfluorosilane using carbon tetrachloride and trimethylchlorosilane. Drawbacks of other methods as well as advantages of the proposed method are discussed.

The method is directly applicable to plasma and faeces samples, has a short gas Chromatographic run time due to the use of a backflush technique and is suitable for automation. Its lowest quantifiable limit for plasma and faeces is 10 μg/l and 1 μg/g fluoride respectively, using 1.0 ml or 1.0 g samples. Preliminary pharmacokinetic parameters of fluoride obtained in two subjects are reported.


Internal Medicine Fluoride Pharmacokinetic Parameter Faeces Sample Tetrachloride 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Rich C, Ensinck J. Effect of sodium fluoride on calcium metabolism of human beings. Nature 1961;191;184.PubMedGoogle Scholar
  2. 2.
    Van Kesteren RG. De behandeling van osteoporose met natriumfluoride. Utrecht: Rijksuniversiteit Utrecht, 1978. 166 pp. Dissertation.Google Scholar
  3. 3.
    Husdan H, Vogl R, Oreopoules D, Gryfe C, Rappoport A. Serum IonicFluoride: Normal Range and Relationship to Age and Sex. Clin Chem 1976;22:1884–8.PubMedGoogle Scholar
  4. 4.
    Fry BW, Taves DR. Serum fluoride analysis with the fluoride electrode. J Lab Clin Med 1970;75:1020–5.PubMedGoogle Scholar
  5. 5.
    Fresen JA, Cox FH, Witter MJ. The determination of fluoride in biological materials by means of gaschromatography. Pharm Weekbl 1968;103:909–14.PubMedGoogle Scholar
  6. 6.
    Bock R, Semmler HJ. Abtrennung und Bestimmung des Fluorid-Ions mit Hilfe siliciumorganischer Verbindungen. Z Anal Chem 1967;230:161–84.CrossRefGoogle Scholar
  7. 7.
    Rowland M, Tucker G. Symbols in Pharmacokinetics. J Pharmacokinet Biopharm 1980;8:497–507.CrossRefPubMedGoogle Scholar
  8. 8.
    Wagner JG. Linear Pharmacokinetic Equations Allowing Direct Calculation of Many Needed Pharmacokinetic Parameters from the Coefficients and Exponents of Polyexponential Equations Which Have Been Fitted to the DATA. J Pharmacokinet Biopharm 1976;4:443–67.PubMedGoogle Scholar
  9. 9.
    Ekstrand J, Ehrnebo M, Boréus L. Fluoride bioavailability after intravenous and oral administration: Importance of renal clearance and urine flow. Clin Pharmacol Ther 1978;23:329–37.PubMedGoogle Scholar

Copyright information

© Royal Dutch Association for Advancement of Pharmacy 1984

Authors and Affiliations

  • J. H. Glerum
    • 1
    • 2
  • A. van Dijk
    • 1
  • S. W. Klein
    • 1
  1. 1.Department of Hospital PharmacyState University Hospital UtrechtGV UtrechtThe Netherlands
  2. 2.Department of Biopharmaceutics, Biopharmaceutical LaboratoryState University of UtrechtGV UtrechtThe Netherlands

Personalised recommendations