Demonstration of mother-to-infant transmission ofStaphylococcus aureus by pulsed-field gel electrophoresis
We assessed mother-to-infant transmission ofStaphylococcus aureus. Anterior nares swabs of 466 pregnant women, vaginal swabs of 305 of these women and anterior nares swabs of 305 6-day-old infants were examined for the presence ofS. aureus. The results showed that 7.5% of the vaginal swabs from the pregnant women and 10.1% of the anterior nares swabs from the infants were positive forS. aureus. Six of the 466 pregnant women (1.3%) and 12 of the 305 infants (3.9%) carried methicillin-resistantS. aureus (MRSA) in the anterior nares site, but none of the vaginal specimens were positive for MRSA. Analysis ofSmaI digested chromosomal DNA analysis using pulsed-field gel electrophoresis (PFGE) showed that methicillin-sensitiveS. aureus (MSSA) strains obtained from four pairs of pregnant women and their infants were completely identical, which strongly suggesting mother-to-infant transmission ofS. aureus.
This study elucidated the prevalence ofS. aureus carriage among pregnant women and newborn infants. Mother-to-infant infection ofS. aureus was demonstrated phenotypically and genetically. PFGE is a useful tool to detect infection routes including mother-to-infant-infection.
Key wordsMother-child relations Bacterial infection Molecular epidemiology
minimal inhibitory concentration
polymerase chain reaction
pulsed-field gel electrophoresis
toxic shock syndrome toxin-1
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