Experientia

, Volume 49, Issue 10, pp 906–909 | Cite as

PCR jumping in clones of 30-million-year-old DNA fragments from amber preserved termites (Mastotermes electrodominicus)

  • R. DeSalle
  • M. Barcia
  • C. Wray
Research Articles

Abstract

DNA from 30-million-year-old amber preserved termites (Mastotermes electrodominicus) was PCR amplified with nuclear ribosomal RNA small subunit primers and cloned into the TA vector (INVITROGEN). We obtained several classes of recombinant clones as a result. AuthenticMastotermes electrodominicus clones were identified. The source of other classes of clones was identified as contaminants of the ancient DNA template. Several of the clones appeared to be chimeric in structure with half of the clone identical to the termite sequence and the other half identical to contaminant sequences. The phenomenon of PCR jumping was identified as a possible source for the chimeric clones.

Key words

Polymerase chain reaction ancient DNA termite relationships Mastotermes electrodominicus 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature

  1. 1.
    Paabo, S., Nature314 (1985) 644.PubMedGoogle Scholar
  2. 2.
    Doran, H. G., Dickel, D. N., Ballinger, W. E., Agee, P. J., and Laipis, W. W., Nature323 (1986) 803.PubMedGoogle Scholar
  3. 3.
    Lawlor, D., Dickel, C., Hauswirth, W., and Parham, P., Nature349 (1991) 785.PubMedGoogle Scholar
  4. 4.
    Higuchi, R., Dowman, B., Freiberger, M., Ryder, O., and Wilson, A. C., Nature312 (1984) 282.PubMedGoogle Scholar
  5. 5.
    Thomas, R. H., Schaffner, W., Wilson, A. C., and Paabo, S., Nature340 (1989) 465.PubMedGoogle Scholar
  6. 6.
    Thomas, W. K., Paabo, S., Villablanca, F. X., and Wilson, A. C., J. molec. Evol.31 (1990) 101.PubMedGoogle Scholar
  7. 7.
    Patton, J. L., and Smith, M. F., Evolution46 (1992) 148.Google Scholar
  8. 8.
    Golenberg, E. M., Giannasi, D. E., Clegg, M. T., Smiley, C. J., Durbin, M., Henderson, D., and Zurawski, G., Nature344 (1990) 656.PubMedGoogle Scholar
  9. 9.
    Hagelberg, E., Sykes, B., and Hedges, R., Nature342 (1989) 485.PubMedGoogle Scholar
  10. 10.
    Yokoi, T., Aoki, Y., and Sagisaki, K., Zs. Rechtsmedizin102 (1989) 323.Google Scholar
  11. 11.
    Williams, S. R., Longmire, J. L., and Beck, L. A., Am. J. Anthropol.812 (1990) 318.Google Scholar
  12. 12.
    Cooper, A., Mourer-Chauvire, C., Chambers, G. K., von Haeseler, A., Wilson, A. C., and Paabo, S., Proc. natl Acad. Sci. USA89 (1992) 8741.PubMedGoogle Scholar
  13. 13.
    Janeczewski, D. N., Yuhki, N., Gilbert, D. A., Jefferson, G. T., and O'Brien, S. J., Proc. natl Acad. Sci. USA89 (1992) 9769.PubMedGoogle Scholar
  14. 14.
    Cano, R. J., Poinar, H., and Poinar, G. O., Med. Sci. Res.20 (1992) 249.Google Scholar
  15. 15.
    Cano, R. J., Poinar, H., and Poinar, G. O., Med. Sci. Res.20 (1992) 619.Google Scholar
  16. 16.
    Cano, R. J., Poinar, H., Pieniazek, N., Aftrim, A., and Poinar, G. Jr, Nature363 (1993) 536.PubMedGoogle Scholar
  17. 17.
    DeSalle, R., Gatesy, J., Wheeler, W., and Grimaldi, D., Science257 (1992) 1933.PubMedGoogle Scholar
  18. 18.
    Maniatis, T., Fritsch, E. F., and Sambrook, J., Molecular Cloning: A Laboratory Manual. Cold Spring Harbour Press, Cold Spring Harbour, New York 1982.Google Scholar
  19. 19.
    Swofford, D. L., Phylogenetic Analysis Using Parsimony (PAUP). Illinois Natural History Survey, 1990.Google Scholar
  20. 20.
    Higuchi, R., and Wilson, A. C., Fedn Proc.43 (1984) 1557.Google Scholar
  21. 21.
    Paabo, S., Irwin, D. M., and Wilson, A. C., J. biol. Chem.265 (1990) 4718.PubMedGoogle Scholar
  22. 22.
    Bradley, R. D., Bull, J. J., and Hillis, D. M., personal communication, 1992.Google Scholar

Copyright information

© Birkhäuser Verlag 1993

Authors and Affiliations

  • R. DeSalle
    • 1
  • M. Barcia
    • 1
  • C. Wray
    • 1
    • 2
  1. 1.Department of EntomologyAmerican Museum of Natural HistoryNew YorkUSA
  2. 2.Department of Geology and GeophysicsYale UniversityNew HavenUSA

Personalised recommendations