Experientia

, Volume 42, Issue 2, pp 200–201 | Cite as

Makisterone A and 24-methylenecholesterol from the ovaries of the honey bee,Apis mellifera L

  • M. F. Feldlaufer
  • J. A. Svoboda
  • E. W. HerbertJr
Short Communications Ethology, Ecology, Natural Product Chemistry

Summary

Makisterone A, a 28-carbon ecdysteroid (molting hormone) has been isolated from the ovaries of queen bees. Analysis by reversed-phase and silica high performance liquid chromatography (HPLC) in conjunction with a radioimmune assay (RIA) revealed about 11 ng of makisterone A present per gram of ovaries on a fresh weight basis. No C27 ecdysteroids were detected. The predominant neutral sterol present was 24-methylenecholesterol.

Key words

Ecdysteroids molting hormones makisterone A HPLC RIA honey bee ovaries Apis mellifera neutral sterols 24-methylenecholesterol 

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Copyright information

© Birkhäuser Verlag Basel 1986

Authors and Affiliations

  • M. F. Feldlaufer
    • 1
  • J. A. Svoboda
    • 1
  • E. W. HerbertJr
    • 2
  1. 1.Insect Physiology LaboratoryARS, USDABeltsvilleUSA
  2. 2.Bioenvironmental Bee LaboratoryARS, USDABeltsvilleUSA

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