Experientia

, Volume 45, Issue 11–12, pp 1124–1128 | Cite as

Identification of juvenile-hormone-binding proteins on blotted electropherograms using tritiated juvenile hormones

  • J. R. Wiśniewski
Research Articles
Received:
Accepted:

Summary

An electroblotting method has been developed to identify juvenile-hormone-binding proteins (JHBPs) of insects. JHBPs were separated by means of one- or two-dimensional native PAGE and then transferred onto ion-exchange membranes. Following incubation in buffer containing3H-JH, the hormone-binding proteins could be detected by autoradiography or3H-scanning of the blots. InG. mellonella andL. migratoria hemolymph, respectively, two and five electrophoretically different JHBP species were detected.

Key words

Juvenile hormone binding protein juvenile hormone electroblotting Galleria mellonella Locusta migratoria 
This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Koeppe, J. K., and Kovalick, G. E., in: Biochemical Actions of Hormones, vol. 13, p. 265. Ed. G. Litwack. Academic Press, New York 1986.Google Scholar
  2. 2.
    Goodman, W. G., in: Endocrinology of Insects, p. 147. Eds R. G. H. Downer and H. Laufer. Alan R. Liss. Inc., New York 1983.Google Scholar
  3. 3.
    Engelmann, F., Molec. cell. Endocr.24 (1981) 103.Google Scholar
  4. 4.
    Roberts, P. E., and Wyatt, G. R., Molec. cell. Endocr.31 (1983) 53.Google Scholar
  5. 5.
    Wiśniewski, J. R., and Kochman, M., FEBS Lett.171 (1984) 127.Google Scholar
  6. 6.
    Roberts, P. E., and Jefferies, L. S., Archs Insect Biochem. Physiol.3 (1986) 7.Google Scholar
  7. 7.
    Wiśniewski, J. R., Wawrzeńczyk, C., Prestwich, G. D., and Kochman, M., Insect Biochem.18 (1988) 29.Google Scholar
  8. 8.
    Haeuptle, M. T., Aubert, M., Dijane, J., and Kraehenbuhl, J. P., J. biol. Chem.129 (1983) 305.Google Scholar
  9. 9.
    Fernadez-Pol, J. A., FEBS Lett.143 (1982) 86.Google Scholar
  10. 10.
    Lin, P. H., Selinfreund, R., and Wharton, W., Analyt. Biochem.167 (1987) 128.Google Scholar
  11. 11.
    Sakai, S., and Murakami, H., Analyt. Biochem.167 (1987) 406.Google Scholar
  12. 12.
    Hossenlopp, P., Seurin, D., Segovia-Quinson, B., Hardouin, S., and Binoux, M., Analyt. Biochem.154 (1986) 138.Google Scholar
  13. 13.
    Hubbard, M. J., and Klee, C. B., J. biol. Chem.262 (1987) 15062.Google Scholar
  14. 14.
    Prestwich, G. D., Eng, W.-S., Robles, S., Vogt, R. G., Wiśniewski, J. R., and Wawrzeńczyk, C., J. biol. Chem.263 (1988) 1398.Google Scholar
  15. 15.
    Ozyhar, A., Wiśniewski, J. R., Sehnal, F., and Kochman, M., Insect Biochem.13 (1983) 435.Google Scholar
  16. 16.
    Alwine, J. C., Kemp, D. J., and stark, G. R., Proc. natl Acad. Sci. USA74 (1977) 5350.Google Scholar
  17. 17.
    Symington, J., Green, M., and Brachmann, K., Proc. natl Acad. Sci. USA78 (1981) 177.Google Scholar
  18. 18.
    Davis, B. J., Ann. N. Y. Acad. Sci.121 (1964) 404.Google Scholar
  19. 19.
    de Bruijn, S. M., Koopmanschap, A. B., and de Kort, C. A. D., Physiol. Ent.11 (1986) 7.Google Scholar
  20. 20.
    Giese, C., Spindler, K. D., and Emmerich, H., Z. Naturforsch.32c (1977) 158.Google Scholar
  21. 21.
    Beisiegel, U., Electrophoresis7 (1986) 1.Google Scholar
  22. 22.
    Ożyhar, A., and Kochman, M., Eur. J. Biochem.162 (1987) 675.Google Scholar
  23. 23.
    Ożyhar, A., and Kochman, M., Experientia42 (1986) 1276.Google Scholar
  24. 24.
    Roberts, P. E., Archs Insect Biochem. Physiol.2 (1985) 351.Google Scholar
  25. 25.
    de Kort, C. A. D., and Koopmanschap, A. B., Experientia43 (1987) 904.Google Scholar
  26. 26.
    de Kort, C. A. D., Koopmanschap, A. B., and Ermens, A. A. M., Insect Biochem.14 (1984) 619.Google Scholar

Copyright information

© Birkhäuser Verlag 1989

Authors and Affiliations

  • J. R. Wiśniewski
    • 1
  1. 1.III. Zoologisches Institut-EntwicklungsbiologieUniversität GöttingenGöttingenGermany

Personalised recommendations