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Experientia

, Volume 40, Issue 11, pp 1234–1240 | Cite as

Immune-mediated glomerulonephritis induced by mercuric chloride in mice

  • S. Eneström
  • P. Hultman
Article

Summary

The BALB/c mouse developed mesangial deposits of immune constituents and light microscopical changes characteristic of immune complex glomerulonephritis after 8 weeks' treatment with mercuric chloride given by s.c. injection. There were no signs of linear of granular immune deposits along the glomerular capillary basement membrane after 2 or 8 weeks. The antigen could not be identified. No antibodies to nuclear or renal structures were found. Using a histochemical method (silver amplification) mercury was detected by light and electron microscopy in tubular and glomerular structures. Mercury was present in secondary lysosomes of the mesangial cells after eight weeks of mercury poisoning.

Key words

Mercuric chloride BALB/c mice immunofluorescence silver amplification 

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References

  1. 2.
    Berlin, M., Mercury, in: Handbook on the Toxicology of Metals, pp. 503–539. Eds L. Friberg, G. F. Nordberg and V. B. Vouk, Elsevier/North-Holland Biomedical Press, Amsterdam/New York/Oxford 1979.Google Scholar
  2. 3.
    Bellon, B., Capron, M., Druet, E., Verroust, P., Vial, M.-C., Sapin, C., Girard, J. F., Foidart, M. M., Mahieu, P., and Druet, P. Mercuric chloride induced autoimmune disease in Brown-Norway rats: sequential search for anti-basement membrane antibodies and circulating immune complexes. Eur. J. clin. Invest.12 (1982) 127–133.PubMedGoogle Scholar
  3. 4.
    Bernaudin, J. F., Druet, E., Druet, P., and Masse, R., Inhalation or ingestion of organic or inorganic mercurials produces autoimmune disease in rats. Clin. Immun. Immunopath.20 (1981) 129–135.CrossRefPubMedGoogle Scholar
  4. 5.
    Clarkson, T. W., The pharmacology of mercury compounds. A. Rev. Pharmac.12 (1972) 375–406.CrossRefGoogle Scholar
  5. 6.
    Danscher, G., and Schröder, H. D., Histochemical demonstration of mercury induced changes in rat neurons. Histochemistry60 (1979) 1–7.CrossRefPubMedGoogle Scholar
  6. 7.
    Danscher, G., Histochemical demonstration of heavy metals. Histochemistry71 (1981) 1–16.PubMedGoogle Scholar
  7. 8.
    Druet, E., Sapin, C., Günther, E., Feingold, N., and Druet, P., Mercuric chloride-induced anti-glomerular basement membrane antibodies in the rat. Genetic control. Eur. J. Immun.7 (1977) 348–351.Google Scholar
  8. 9.
    Druet, E., Sapin, C., Fournie, G., Mandet, C., Günther, E., and Druet, P., Genetic control of susceptibility to mercury-induced nephritis in various strains. Clin. Immun. Immunopath.25 (1982) 203–212.CrossRefPubMedGoogle Scholar
  9. 10.
    Druet, P., Druet, E., Potdevin, F., and Sapin, C., Immune type glomerulonephritis induced by HgCl2 in the Brown Norway rat. Ann. Immun. (Inst. Pasteur)129 C (1978) 777–792.Google Scholar
  10. 11.
    Druet, P., Ayed, K., Bariety, J., Bernaudin, J. F., Druet, E., Girard, J. F., Hinglais, N., and Sapin, C., Experimental immune glomerulonephritis induced in the rat by mercuric chloride, in: Advances in Nephrology, pp. 321–342. Eds J. Hamburger et al. Year Book Medical Publisher 15, London 1979.Google Scholar
  11. 12.
    Druet, P., Teychenne, P., Mandet, C., Bascou, C., and Druet, E., Immune-type glomerulonephritis induced in the Brown-Norway rat with mercury-containing pharmaceutical products. Nephron28 (1981) 145–148.PubMedGoogle Scholar
  12. 13.
    Eneström, S., Avantages des coupes semi-fines des biopsies rénales incluses en paraffine et réalisées par coteaux en verre au large tranchant. Archs. Anat. Cytol. Path.29 (1981) 317–319.Google Scholar
  13. 14.
    Eneström, S., and Hultman, P., Mercury induced glumerulonephritis in mice. Abstract, 6th World Congress of Academic and Environmental Pathology, Miami Beach, Florida, September 2–7, 1984.Google Scholar
  14. 15.
    Fournié, G. J., Lulé, J., Gayral-Taminh, M., Mignon-Conté, M., Conté, J. J., Bellon, B., Druet, E., Hirsch, F., Sapin, C., Druet, P., Izui, S., and Lambert, P. H., Immunological pathogenesis of experimental glomerulonephritis induced by some infectious and toxic agents. Contr. Microbiol. Immun.7 (1983) 43–51.Google Scholar
  15. 16.
    Hinglais, N., Druet, P., Grossetete, J., Sapin, C., and Bariéty, J., Ultrastructural study of nephritis induced in Brown-Norway rats by mercuric chloride. Lab. Invest.41 (1979) 150–159.PubMedGoogle Scholar
  16. 17.
    Hirsch, J., Couderc, J., Sapin, C., Fournié, G., and Druet, P., Polyclonal effect of HgCl2 in the rat, its possible role in an eyperimental autoimmune disease. Eur. J. Immun.12 (1982) 620–625.Google Scholar
  17. 18.
    Hultman, P., and Eneström, S., Experimental Hg-nephropathy: correlation between histochemistry and analytical EM. J. Ultrastruct. Res.85 (1983) 118–119.Google Scholar
  18. 19.
    Lelental, M., and Gysling, H. J., Tellurium Physical Development (TPD): A dry non-silver amplification process. J. Photogr. Sci.28 (1980) 209–215.Google Scholar
  19. 20.
    Makker, S. P., and Aikawa, M., Mesangial glomerulonephropathy with deposition of IgG, IgM and C3 induced by mercuric chloride: A new model. Lab. Invest.41 (1979) 45–50.PubMedGoogle Scholar
  20. 21.
    Rodin, A. E., and Crowson, C. N., Mercury nephrotoxicity in the rat 1. Factors influencing the localization of the tubular lesions. Am. J. Path.41 (1962) 297–307.PubMedGoogle Scholar
  21. 22.
    romano-Franco, A. A., Turiello, M., Albini, B., Ossi, E., Milgrom, F., and Giuseppe, A. A., Anti-basement membrane antibodies and antigen-antibody complexes in rabbits injected with mercuric chloride. Clin. Immun. Immunopath.9 (1978) 464–481.CrossRefPubMedGoogle Scholar
  22. 23.
    Sapin, C., Druet, E., and Druet, P., Induction of anti-glomerular basement membrane antibodies in the Brown-Norway rat by mercuric chloride. Clin. exp. Immun.28 (1977) 173–179.PubMedGoogle Scholar
  23. 24.
    Sapin, C., Mandet, C., Druet, G., Günther, G., and Druet P., Immune complex type disease induced by HgCl: genetic control of susceptibility. Transpl. Proc.13 (1981) 1404–1406.Google Scholar
  24. 25.
    Schreiner, G. A., and Maher, J. F., Toxic Nephropathy. Am. J. Med.38 (1965) 404–449.CrossRefGoogle Scholar
  25. 26.
    Siegel, S., The Randomization test for two independent samples, in: Non-parametric statistics for the behavioral sciences, pp. 152–156. Ed. S. Siegel. McGraw-Hill, New York 1956.Google Scholar
  26. 27.
    Stejskal, J., Acute renal insufficiency in intoxication with mercury compounds III. Pathological findings. Acta med. scand.177 (1965) 75–84.PubMedGoogle Scholar
  27. 28.
    Thaw, H. H., Forslid, J., Hamberg, H., and Hed, J., The effect of reactive oxygen metabolites (ROM) on the attachment and indigestion phase of C3b- and IgG-mediated phagocytosis by macrophages. Acta path. microbiol. immun. scand.92 (1984) 1–8.Google Scholar
  28. 29.
    Weening, J. J., Fleuren, G. J., and Hoedemaeker, Ph. J., Demonstration of Antinuclear antibodies in mercuric-chloride-induced glomerulopathy in the rat. Lab. Invest.39 (1978) 405–411.PubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag 1984

Authors and Affiliations

  • S. Eneström
    • 1
  • P. Hultman
    • 1
  1. 1.Department of Pathology ILinköping UniversityLinköping(Sweden)

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