Experientia

, Volume 42, Issue 11–12, pp 1234–1235 | Cite as

A rapid HPLC method for determination of adenylate energy charge

  • A. Viarengo
  • A. Secondini
  • P. Scoppa
  • M. Orunesu
Short Communications

Summary

A simple procedure is described for separation and analysis of adenine nucleotides in tissue extracts, utilizing anion exchange HPLC. Determination of AMP, ADP, and ATP takes 10 min per sample.

Key words

HPLC adenine nucleotide evaluation adenylate energy charge stress index 

References

  1. 3.
    Livingstone, D.R., Mar. Pollut. Bull.13 (1982) 261.Google Scholar
  2. 4.
    Atkinson, D.E., and Walton, G.M., J. biol. Chem.242 (1967) 3238.Google Scholar
  3. 5.
    Ivanovici, A.M., Helgoländer Wiss. Meeresunters.33 (1980) 556.Google Scholar
  4. 6.
    Pogolotti, A.L., and Santi, D.V., Analyt. Biochem.126 (1982) 335.Google Scholar
  5. 7.
    Parris, N.A., Instrumental Liquid Chromatography, 2nd edn. Elsevier, New York 1984.Google Scholar
  6. 8.
    Wijsman, T.C.M., J. cell. comp. Physiol.107 (1976) 129.Google Scholar

Copyright information

© Birkhäuser Verlag 1986

Authors and Affiliations

  • A. Viarengo
    • 1
  • A. Secondini
    • 2
  • P. Scoppa
    • 2
  • M. Orunesu
    • 1
  1. 1.Istituto di Fisiologia Generale dell'Università di GenovaGenova(Italy)
  2. 2.Centro Ricerche Energia AmbienteENEALa Spezia(Italy)

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