Experientia

, Volume 33, Issue 6, pp 762–763 | Cite as

Specific immunostaining of CCK cells by use of synthetic fragment antisera

  • Julia M. Polak
  • A. G. E. Pearse
  • M. Szelke
  • S. R. Bloom
  • D. Hudson
  • P. Facer
  • Alison M. J. Buchan
  • M. G. Bryant
  • N. Christophodes
  • I. MacIntyre
Article

Summary

Antibodies to the central fragments 9–20 dodecapeptide sequence of CCK were used for specific immunostaining of the CCK cells of the mammalian gut. The use of high specific antibodies to synthetic fragment, essential when there is a possibility of immunochemical cross reactions between antisera and hormones of similar molecular structure provides the key to increased understanding of the nature and relationships of peptide hormones.

Keywords

Peptide Molecular Structure Specific Antibody Cross Reaction Peptide Hormone 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Birkhäuser Verlag 1977

Authors and Affiliations

  • Julia M. Polak
    • 1
  • A. G. E. Pearse
    • 1
  • M. Szelke
    • 1
  • S. R. Bloom
    • 1
  • D. Hudson
    • 1
  • P. Facer
    • 1
  • Alison M. J. Buchan
    • 1
  • M. G. Bryant
    • 1
  • N. Christophodes
    • 1
  • I. MacIntyre
    • 1
  1. 1.Department of Histochemistry and Medicine, and Endocrine Unit, Royal Postgraduate Medical SchoolHammersmit HospitalLondon(England)

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